研究目的
To develop a DFLC-based biosensor for detecting and quantitating bovine serum albumin (BSA) using frequency-dependent dielectric properties.
研究成果
The study successfully demonstrated the use of DFLC's unique dielectric properties for protein quantitation, establishing a quantitative protein assay method with potential applications in biosensing.
研究不足
The study was limited to the detection and quantitation of BSA, and the LOD was of the order of 10^-6 g/ml. Further improvement of the LOD is necessary for broader biochemical applications.
1：Experimental Design and Method Selection:
The study utilized a DFLC-based biosensor to analyze the dielectric spectra of DFLC in the presence of BSA at various concentrations.
2：Sample Selection and Data Sources:
BSA was used as the protein standard, immobilized on DMOAP-modified glass substrates.
3：List of Experimental Equipment and Materials:
ITO-coated glass slides, DMOAP, BSA, DFLC, Agilent E4980 A high-precision LCR meter, Linkam T95-PE temperature controller, Olympus BX51 polarizing optical microscope.
4：Experimental Procedures and Operational Workflow:
BSA was immobilized on DMOAP-modified glass slides, and DFLC was injected into the LC cells. Dielectric spectra were obtained at various BSA concentrations.
5：Data Analysis Methods:
The dielectric permittivity difference between high- and low-frequency regimes was analyzed to correlate with BSA concentration.
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BSA
Lyophilized powder, 66.43 kDa
Sigma–Aldrich
Used as the protein standard for detection and quantitation.
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Agilent E4980 A high-precision LCR meter
E4980 A
Agilent
Used for obtaining dielectric spectra.
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Olympus BX51 polarizing optical microscope
BX51
Olympus
Used for observing the optical textures of LC cells.
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ITO-coated glass slides
18.3 mm × 16.7 mm, 1.1 mm thickness
Chipset Technology Co., Ltd.
Used as substrates for the LC cells.
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DMOAP
Sigma–Aldrich
Used as the vertical alignment agent for DFLC.
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DFLC
Jiangsu Hecheng Technology Co., Ltd.
Used as the sensing medium in the biosensor.
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Linkam T95-PE temperature controller
T95-PE
Linkam
Used for temperature control during dielectric measurements.
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