研究目的
Investigating the detection of pyrophosphate (PPi) through a two-photon absorption (TPA) benzoxazole-based fluorescence probe BN using Cu2+ displacing method in situ system in aqueous medium.
研究成果
The study successfully designed and synthesized a two-photon absorption (TPA) benzoxazole-based fluorescence probe BN for detecting PPi through Cu2+ displacing method in situ system in aqueous medium, verified by mass spectra and theoretical calculations, and applied in TPA cells imaging.
研究不足
The study's limitations include the need for further optimization of the probe's sensitivity and selectivity in complex biological environments.
1：Experimental Design and Method Selection:
The study involved the design and synthesis of a two-photon absorption (TPA) benzoxazole-based fluorescence probe BN for detecting PPi through Cu2+ displacing method in situ system.
2：Sample Selection and Data Sources:
The probe was tested in aqueous medium and applied in TPA cells imaging.
3：List of Experimental Equipment and Materials:
The synthesis involved various chemical compounds and solvents, with characterization techniques including mass spectra and theoretical calculations.
4：Experimental Procedures and Operational Workflow:
The probe's interaction with PPi was verified through mass spectra and theoretical calculations, followed by application in TPA cells imaging.
5：Data Analysis Methods:
The study utilized time-dependent density functional theory (TD-DFT) calculations for theoretical analysis.
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