研究目的
To improve the detection sensitivity and spectral quality of probe molecules on surface-enhanced Raman scattering (SERS) substrates by using the molecular template reagent (MTR) technique.
研究成果
The MTR technique significantly improves the detection sensitivity of probe molecules on SERS substrates by adjusting their orientations, leading to an order of magnitude increase in the limit of detection (LOD). This method offers a promising approach for ultrasensitive analyses in various fields.
研究不足
The study focuses on the effect of MTRs on the orientation of probe molecules and their SERS signals. The applicability of the MTR technique to a wider range of molecules and substrates needs further investigation.
1：Experimental Design and Method Selection:
The study employs the MTR technique based on SERS surface selection rules to adjust the orientations of probe molecules on SERS substrates.
2：Sample Selection and Data Sources:
Probe molecules (p-aminobenzenethiol and 4-mercaptobenzoic acid) and MTRs (1-butanethiol, 1-hexanethiol, 1-octanethiol, 1-decanethiol, and 1-dodecanethiol) are used.
3：List of Experimental Equipment and Materials:
Ag NPs are prepared and deposited on glass slides. SEM and UV–vis spectroscopy are used for characterization.
4：Experimental Procedures and Operational Workflow:
The substrates are prepared by depositing Ag NPs on glass slides, followed by adsorption of probe molecules and MTRs. SERS spectra are recorded.
5：Data Analysis Methods:
The SERS intensities are analyzed to determine the effect of MTRs on the detection sensitivity of probe molecules.
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Shimadzu UV-3600 spectrophotometer
UV-3600
Shimadzu
Used to record UV–vis spectra.
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JEOL JSM-6700F field-emission scanning electron microscope
JSM-6700F
JEOL
Used to obtain morphology images of the samples.
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Silver nitrate
AgNO3
Sigma–Aldrich Chemical Co., Inc.
Used in the preparation of Ag NPs for SERS substrates.
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Poly(diallyldimethylammonium chloride)
PDDA
Sigma–Aldrich Chemical Co., Inc.
Used in the layer-by-layer self-assembly technique for depositing Ag NPs on glass slides.
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Trisodium citrate dihydrate
Sigma–Aldrich Chemical Co., Inc.
Used in the synthesis of Ag NPs.
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1-butanethiol
BT
Sigma–Aldrich Chemical Co., Inc.
Used as a molecular template reagent (MTR) to adjust the orientations of probe molecules.
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1-hexanethiol
HT
Sigma–Aldrich Chemical Co., Inc.
Used as a molecular template reagent (MTR) to adjust the orientations of probe molecules.
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1-octanethiol
OT
Sigma–Aldrich Chemical Co., Inc.
Used as a molecular template reagent (MTR) to adjust the orientations of probe molecules.
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1-decanethiol
DT
Sigma–Aldrich Chemical Co., Inc.
Used as a molecular template reagent (MTR) to adjust the orientations of probe molecules.
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1-dodecanethiol
Sigma–Aldrich Chemical Co., Inc.
Used as a molecular template reagent (MTR) to adjust the orientations of probe molecules.
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p-aminobenzenethiol
PATP
Sigma–Aldrich Chemical Co., Inc.
Used as a probe molecule in SERS detection.
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4-mercaptobenzoic acid
4-MBA
Sigma–Aldrich Chemical Co., Inc.
Used as a probe molecule in SERS detection.
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Ultrapure water
Milli-Q plus system (Millipore Co.)
Used in all aqueous solutions and rinsing procedures.
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Horiba–Jobin Yvon LabRAM ARAMIS system
LabRAM ARAMIS
Horiba–Jobin Yvon
Used to obtain SERS spectra.
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