研究目的
To establish and evaluate a model for tracking polymeric particles in fluorescently stained biological material, specifically in biofilms, using a blue fluorescent labeling technique that does not interfere with live/dead staining methods.
研究成果
The study successfully established a blue fluorescent labeling technique for tracking polymeric particles in biofilms, complementing existing live/dead staining methods. The AMCA-labeled PLGA particles showed good penetration into biofilms and could be clearly distinguished from bacteria, offering a valuable tool for studying drug delivery systems in biofilm-associated infections.
研究不足
The study notes that the fluorescence of AMCA-labeled particles decreased over time, likely due to hydrolytic degradation, which could limit long-term tracking applications. Additionally, the interaction of SYTO9? with the particles' surface could affect visualization.
1：Experimental Design and Method Selection:
The study involved the covalent labeling of PLGA with AMCA to create blue fluorescent particles. A modified solvent evaporation method was used to prepare micro- and nanoparticles.
2：Sample Selection and Data Sources:
Biofilms of Burkholderia cepacia and Staphylococcus aureus were used to test the penetration and visualization of the particles.
3：List of Experimental Equipment and Materials:
Included PLGA, AMCA, PEG-PLGA, chitosan, and various solvents and reagents for particle preparation and characterization.
4：Experimental Procedures and Operational Workflow:
Particles were prepared, characterized for size and charge, and then applied to biofilms. Penetration and distribution were visualized using confocal laser scanning microscopy.
5：Data Analysis Methods:
Fluorescence intensity measurements, size exclusion chromatography, and SEM were used for particle characterization.
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