研究目的
To design and synthesize two fluorescent probes based on anthracene derivatives for the selective and sensitive detection of biothiols such as cysteine (Cys) and glutathione (GSH) in live cells.
研究成果
The designed fluorescent probes (compound 1-Cu2+ and compound 2-Cu2+) showed good selectivity and sensitivity to GSH and Cys in DMSO solution. The Circular Dichroism responses can distinguish Cys from GSH. The probes have potential application value in living cell imaging and detection due to their high sensitivity, good selectivity, and quick reaction.
研究不足
The study primarily focuses on the detection of Cys and GSH, with less emphasis on other biothiols like homocysteine (Hcy). The probes' performance in more complex biological environments needs further investigation.
1：Experimental Design and Method Selection:
Two compounds (1 and 2) were synthesized from 9-furaldehyde with benzenesulfonamide and p-nitrobenzenesulfonamide, respectively, and then combined with copper ion to form two copper complexes (compound 1-Cu2+ and compound 2-Cu2+). The fluorescence quenching and recovery mechanisms were investigated.
2：Sample Selection and Data Sources:
All amino acids were from Aladdin Chemical Co. Ltd. (Shanghai, China). Stock solutions were prepared in aqueous solutions.
3：List of Experimental Equipment and Materials:
UV-vis titrations were tested using a Shimadzu UV2600. Fluorescence spectra were measured using an Eclipse fluorescence spectrophotometer (Agilent, USA). Circular dichroism was performed using a circular dichroism (CD) spectrometers (Chirascan, UK).
4：Fluorescence spectra were measured using an Eclipse fluorescence spectrophotometer (Agilent, USA). Circular dichroism was performed using a circular dichroism (CD) spectrometers (Chirascan, UK).
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The synthesized compounds were characterized by UV-vis, fluorescence spectra, and Circular Dichroism. The cytotoxicity of the probes was evaluated using MTT assay against human hepatoma cell lines (HepG2 cell).
5：Data Analysis Methods:
The binding constant (Ks) was fitted by a nonlinear least square method. All results were average. To detect significant differences between assays, a non-parametric Kruskal-Wallis test were used with a significance level of 5%.
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