研究目的
To develop an effective fluorescent probe for the selective detection of bisulfite in real samples and living cells due to its association with toxicological effects.
研究成果
The fluorescent probe QPCT was successfully developed for the colorimetric and ratiometric detection of bisulfite with high selectivity and sensitivity. It has been effectively applied in real samples and living cells, demonstrating its potential for monitoring bisulfite in complex systems.
研究不足
The study focuses on the detection of bisulfite in specific real samples and living cells, and the probe's performance in other complex biological systems or environmental samples was not explored.
1：Experimental Design and Method Selection:
The probe QPCT was designed based on a 1,4-addition mechanism for detecting bisulfite.
2：Sample Selection and Data Sources:
A549 cells and real samples (white sugar, brown sugar, crystal sugar) were used.
3：List of Experimental Equipment and Materials:
Varian INOVA 400 spectrometer, Bruker micro TOF-Q mass spectrometer, F-2700 fluorescence spectrometer, Shimadzu UV-1700 spectrophotometer, FE28-Standard pH meter, confocal laser scanning microscope (Olympus FV 1000).
4：0). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Synthesis of QPCT, spectroscopic measurements, cell culture and fluorescence imaging, cytotoxicity assay.
5：Data Analysis Methods:
Fluorescence spectra analysis, UV-Vis absorption spectra analysis, confocal microscopy imaging analysis.
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