1：Experimental Design and Method Selection:

The study employs a competitive electrochemical assay where the measured current values (related to WOR, catalyzed by IrO2 NPs) are inversely proportional to the concentration of BDE-47 in the sample. Magnetic beads (MBs) coated with anti-PBDE antibodies are used to capture BDE-47 and horseradish peroxidase (HRP)-PBDE conjugate to link IrO2 NPs to PBDE.

2：Sample Selection and Data Sources:

Samples include different concentrations of BDE-47 in distilled water.

3：List of Experimental Equipment and Materials:

Instruments used include Allegra 64 R Centrifuge, Biosan TS-100 Thermoshaker, Malvern Zetasizer Nano 26, Autolab PGSTAT 302, hot plate Fisherbrand, Screen Printer DEK248, Tecnai F20 TEM (FEI), Perkin Elmer NexION 300X ICP-MS Spectrometer. Materials include BDE-47, bovine serum albumin (BSA), K2IrCl6, sodium citrate sesquihydrate, NaOH, and phosphate buffer saline (PBS) tablets.

4：Experimental Procedures and Operational Workflow:

The assay involves the synthesis of IrO2 NPs, their conjugation with PBDE-HRP, characterization of the nanoparticles, fabrication of SPCEs, preparation of samples, and performance of the assay through chronoamperometric measurements.

5：Data Analysis Methods:

The current value at 200 s is considered as the analytical signal, which is normalized by dividing the values obtained by the current value of concentrated IrO2 NPs measured under the same conditions.