研究目的
Investigating the therapeutic effects of peptide-modified ZnO nanoparticles on UV-B radiation protection in skin.
研究成果
The study demonstrates that ZnO-60 nanoparticles surface-modified with the amphipathic peptide M9 can reside in the upper skin layers, enter keratinocytes with minimal toxicity, and show efficient photoprotection and minimum phototoxicity. This modification confers stability and reduces aggregation and dissolution of the nanoparticles. Moreover, the nanoparticles do not go into systemic circulation, and are cleared from the skin in seven days. All these features make these nanoparticles a suitable choice for further development as a photoprotective agent.
研究不足
The study is limited to the specific peptide M9 and ZnO nanoparticles of sizes 30 nm, 60 nm, and 100 nm. The in vivo studies were conducted on SKH-1 mice, and the results may not directly translate to human skin. The long-term effects and potential immunogenic responses were not fully explored.
1：Experimental Design and Method Selection:
ZnO nanoparticles of different sizes were functionalized with an amphipathic peptide M9 to study its effect on nanoparticle stabilization and UV photoprotective activity.
2：Sample Selection and Data Sources:
Human keratinocyte HaCaT cells and SKH-1 mice were used for in vitro and in vivo studies, respectively.
3：List of Experimental Equipment and Materials:
Zinc acetate dehydrate, methanol, sodium hydroxide, APTES, FITC, NHS, EDC, Zinquin ethyl ester dye, MTT, M9 peptide, TEM, DLS, FTIR spectroscopy, UV-Vis spectrophotometer, Franz Diffusion Apparatus, SEM, ICP-MS.
4：Experimental Procedures and Operational Workflow:
Synthesis of bare and M9 modified ZnO nanoparticles, characterization, aggregation and dissolution propensity measurement, photocatalytic assay, Franz diffusion assay, in vivo studies, cell culture experiments, genotoxicity assessment, ROS production measurement, SOD assay, cell cycle analysis, ELISA for DNA damage measurement, cytokine profiling.
5：Data Analysis Methods:
Statistical analysis was performed using GraphPad Prism 6 software.
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FTIR spectrometer
NICOLET 380
Thermo Scientific
Chemical characterization
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UV-Vis spectrophotometer
Cary Series
Agilent Technologies
Absorbance measurement
-
SEM
FEI Quanta 200F
FEI
Surface feature analysis
-
ICP-MS
Agilent ICP-MS 7900
Agilent
Elemental analysis
-
Zinc acetate dehydrate
Not provided
Sigma Aldrich
Precursor for ZnO nanoparticle synthesis
-
Methanol
Not provided
SRL Enterprises
Solvent for nanoparticle synthesis
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Sodium hydroxide
Not provided
Sigma Aldrich
Base for nanoparticle synthesis
-
APTES
Not provided
Sigma Aldrich
Surface modification of nanoparticles
-
FITC
Not provided
Sigma Aldrich
Fluorescent labeling
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NHS
Not provided
Sigma Aldrich
Coupling agent
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EDC
Not provided
Sigma Aldrich
Coupling agent
-
Zinquin ethyl ester dye
Not provided
Sigma Aldrich
Zn2+ detection
-
MTT
Not provided
USB, Affymetrix
Cell viability assay
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M9 peptide
Not provided
SBS Genentech, China
Nanoparticle modification
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Franz Diffusion Apparatus
Not provided
Not provided
Transdermal diffusion study
-
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