研究目的
To overcome tumor hypoxia for enhanced photodynamic therapy (PDT) by constructing a two-photon triggered nanoplatform, FCRH nanocomposite, to alleviate tumor hypoxia and enhance photodynamic therapy via generating O2 in situ.
研究成果
The FCRH nanocomposite demonstrated efficient O2 supply, deep penetration, and spatial precision under two-photon irradiation, significantly enhancing PDT efficiency by overcoming tumor hypoxia. This approach provides a promising strategy for tumor therapy with potential for clinical applications.
研究不足
The study primarily focuses on the in vitro and in vivo evaluation of FCRH nanocomposite for PDT enhancement and hypoxia overcoming. Potential limitations include the need for further optimization of the nanocomposite for clinical applications and the exploration of its long-term biocompatibility and toxicity.
1：Experimental Design and Method Selection:
The study involved the design and synthesis of a two-photon excited nanocomposite FCRH, integrating Fe-C3N4, Ru(bpy)3 2+, and HOP for enhanced TP-PDT. The methodology included the fabrication of Fe-C3N4@Ru@HOP (FCRH) nanocomposite and the evaluation of its photocatalytic activity and O2 generation under two-photon irradiation.
2：Sample Selection and Data Sources:
Mammary carcinoma (4T1) cells were used to evaluate the cellular uptake and cytotoxicity of FCRH. In vivo studies were conducted on 4T1-tumor-bearing Balb/C mice.
3：List of Experimental Equipment and Materials:
Instruments included TEM (JEM-2100 (HR)), PXRD (Rigaku MiniFlex 600 X-ray diffractometer), FT-IR spectrophotometer (Perkin Elmer), UV-vis spectrophotometer (Perkin Elmer Lambda Bio40), and a mode-locked Ti: sapphire femtosecond pulsed laser (Chameleon Ultra I, Coherent, America). Materials included Ru(bpy)3 2+, Fe-C3N4, HOP, and various biochemical reagents.
4：Experimental Procedures and Operational Workflow:
The study involved the synthesis of FCRH, characterization of its properties, in vitro and in vivo evaluation of its O2 generation and PDT efficacy, and metabonomics analysis of tumor tissues.
5：Data Analysis Methods:
Data analysis included statistical analyses using Simca P 13.0 software for principal component analysis and Microsoft Excel 2017 for two-tailed Student’s t-test.
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JEM-2100 (HR)
JEM-2100 (HR)
JEOL
Transmission electron microscopy imaging
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Rigaku MiniFlex 600 X-ray diffractometer
MiniFlex 600
Rigaku
X-ray diffraction analysis
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Perkin Elmer UV-vis spectrophotometer
Lambda Bio40
Perkin Elmer
UV-vis absorbance spectra detection
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Ti: sapphire femtosecond pulsed laser
Chameleon Ultra I
Coherent
Photodynamic therapy
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DCS200PC Photon Counting
DCS200PC
Beijing Zolix Instruments Co., Ltd.
Two-photon excited fluorescence intensity recording
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Bruker X-band A200 spectrometer
A200
Bruker
ESR spectra measurement
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Quantum FX microCT
Quantum FX
Perkin Elmer
CT scanning
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Confocal laser scanning microscope
Nikon C1-si TE2000
Nikon
Fluorescence microscopy imaging
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IVIS imaging systems
Perkin Elmer
In vivo imaging experiments
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Flow cytometry
BD FACSAria TM III
BD
Cell death assessment
-
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