研究目的
To determine whether sO2 mapping shows serial changes in the kidney following ischemia-induced AKI and to compare this mapping to histological examination and 24 h creatinine clearance (CCr) and serum blood urea nitrogen (BUN) level.
研究成果
PAI provided a sensitive measure of the change in sO2 in the acute phase of AKI, with the sO2 at 24 h after AKI being predictive of renal function in the chronic phase.
研究不足
The small number of animals in each group and the diversity of kidney function among animals within each group. The use of PAI over the incision performed on the back for AKI-induction, rather than completely noninvasive imaging.
1：Experimental Design and Method Selection:
The study used a renal ischemia-reperfusion model in C57Bl/6 mice to induce mild and severe AKI. PAI was used to measure renal oxygen saturation (sO2) at various time points post-ischemia.
2：Sample Selection and Data Sources:
Thirty-five male C57Bl/6N mice were used, divided into mild and severe AKI groups based on the duration of ischemia.
3：List of Experimental Equipment and Materials:
A Vevo LAZR small-animal US and PA imaging system with a LZ-550 linear-array transducer was used for imaging.
4：Experimental Procedures and Operational Workflow:
AKI was induced by clamping the left renal pedicle for 35 or 50 minutes. PAI was performed before injury and at 4, 8, 12, 24, and 48 h after ischemia-reperfusion.
5：Data Analysis Methods:
Statistical analyses were performed using GraphPad software, with Mann-Whitney U-test and Dunn’s multiple comparisons used for between-group differences.
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