研究目的
The use of Raman spectroscopy to detect pathological changes in the kidney tissues.
研究成果
The conducted research showed the Raman signals of the pig kidney cortex fixed in formalin have an insignificant contribution of formaldehyde due to differences in the density of medulla and cortex substances. The Raman signals of the medulla and the cortex remain unchanged for both (unfixed and fixed in formalin) states. Spectral differences of human kidney clear-cell adenocarcinoma are probably caused by the presence of mitochondria high concentrations in the cytoplasm of cancer cells associated with intensity differences at 876 сm-1, 975 сm-1 and 1335 сm-1.
研究不足
The study was limited to the spectral analysis of kidney tissues and its neoplasms using Raman spectroscopy. The interaction effect of formalin and kidney tissues on the spectral characteristics was also considered.
1：Experimental Design and Method Selection:
Raman spectroscopy was used as the research method, which allowed for highlighting changes in chemical compounds of the examined samples.
2：Sample Selection and Data Sources:
Samples of the pig kidney tissues were studied as a model medium. Human kidney tissues with pathological neoplasms were also examined.
3：List of Experimental Equipment and Materials:
The experimental setup included a thermally stabilized diode laser, commercial Raman probes, and a spectrograph with a deeply cooled digital camera.
4：Experimental Procedures and Operational Workflow:
Raman spectra were registered in 780-950 nm spectral range, the exposure time was 60 seconds.
5：Data Analysis Methods:
Projection to latent structures (PLS) method was used for the experimental data analysis. The registered signal includes the autofluorescence (AF) and Raman scattering, so a raw spectrum preprocessing was performed for the autofluorescence background removal.
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