摘要： Schiff bases consist of aromatic have been used in various application including biological, inorganic and analytical chemistry. It also has been applied in optical and electrochemical sensors to enable detection of enhance selectivity and sensitivity of the sensors. These compounds might give excellent prospect in sensor. Schiff bases derived from 4-(4- aminostyryl)benzonitrile and three 4-alkoxybenzaldehyde derivatives are synthesis using ethanol as solvent. All compounds were characterized by Fourier transform-infrared spectroscopy (FT-IR), UV-Vis spectrophotometer and Nuclear Magnetic Resonance (NMR). FTIR showed formation of C=N (imine) stretching vibrations at range of 1604 cm-1 to 1609 cm- 1. In UV-vis, π-π* electronic transition of the C=N can be observed at peak range 361 nm. While in the δH NMR the peak of CH=N (Imine) group was found at δ 8.32-8.34 ppm and 13C NMR for three Schiff bases were discovered at δ 158.72, 158.73 and 158.71 ppm. In previous study there are several types of linker that have been used in E-DNA, especially alkanethiol. In this study, three new Schiff base derivatives have been synthesized and characterized due to it promising in sensor which prompted us to apply its as future potential linker in electrochemical DNA sensor.