研究目的
To develop a green approach for the synthesis of silver nanoparticles using aqueous extract of Durio zibethinus seed and to determine its antibacterial, photocatalytic and cytotoxic effects.
研究成果
The green synthesis of DSAgNPs using Durio zibethinus seed extract under sunlight is an environmentally friendly approach that yields effective nanoparticles with antibacterial, photocatalytic, and cytotoxic activities. These nanoparticles have potential applications in water treatment, pharmaceuticals, biomedicine, biosensor, and nanotechnology.
研究不足
The study did not explore the long-term stability of DSAgNPs under various environmental conditions. The antibacterial activity was tested against a limited number of bacterial strains. The cytotoxicity was only preliminary tested against brine shrimp, and further studies on cell-line toxicity and anti-tumor activities are needed.
1：Experimental Design and Method Selection:
The study utilized a green synthesis approach for silver nanoparticles using Durio zibethinus seed extract under sunlight.
2：Sample Selection and Data Sources:
Fresh and healthy durian seeds were acquired from a local market.
3：List of Experimental Equipment and Materials:
Silver nitrate, nutrient broth, Muller Hinton Agar, bacterial cultures, and various characterization instruments like UV-Vis spectrophotometer, SEM, TEM, EDX, XRD, and Zetasizer.
4：Experimental Procedures and Operational Workflow:
Preparation of durian seed extracts, biosynthesis of DSAgNPs, optimization of reaction parameters, characterization of DSAgNPs, antimicrobial activity assessment, photocatalytic activity evaluation, and cytotoxicity testing against Artemia salina.
5：Data Analysis Methods:
UV-Vis spectroscopy for SPR shift, SEM and TEM for morphology and size, EDX for elemental composition, XRD for crystallinity, and Zetasizer for zeta potential.
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