研究目的
To evaluate the ability of multiphoton microscopy (MPM) for label-free detection of brain invasion in meningiomas by combining two-photon excited fluorescence imaging, second-harmonic generation imaging, and spectral imaging.
研究成果
MPM has the ability to morphologically and spectrally identify brain invasion in meningiomas. With the development of probes and optical fiber for in vivo imaging, combined with laser ablation systems, MPM has the potential to provide immediate diagnosis and curative treatment of brain invasive meningiomas.
研究不足
The study is limited by the need for miniaturization and optimization of probes and optical fiber for in vivo imaging. Additionally, the research on multiphoton ablation of brain invasive meningiomas will be emphasized in the future.
1：Experimental Design and Method Selection:
MPM was used for label-free detection of brain invasion in meningiomas. The study combined two-photon excited fluorescence imaging, second-harmonic generation imaging, and spectral imaging.
2：Sample Selection and Data Sources:
Normal brain samples from three patients and meningioma samples from six patients were taken during neurosurgical procedures. Samples were processed according to standard histology procedures.
3：List of Experimental Equipment and Materials:
A Zeiss LSM 510 META system equipped with a mode-locked Ti: sapphire laser was used for MPM imaging. An oil immersion objective was used to focus the excitation beam into specimens.
4：Experimental Procedures and Operational Workflow:
The system had two imaging modes: lambda mode and channel mode. SHG and TPEF signals were gathered and coded green and red color, respectively, to increase contrast.
5：Data Analysis Methods:
A program was developed to depict clear boundary between tumor and normal regions in MPM images using k-means cluster division method, opening operation, and closing operation.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
