1：Experimental Design and Method Selection:

The study used microarc oxidation (MAO) to prepare coatings on titanium surfaces, with Zn and Sr doping to enhance biological activity. In vitro and in vivo tests were conducted to evaluate osteogenic and antibacterial effects.

2：Sample Selection and Data Sources:

Pure TA2 titanium samples were used, cut into specific dimensions. MC3T3-E1 preosteoblasts and S. aureus bacteria were cultured for in vitro assays; New Zealand rabbits were used for in vivo studies.

3：List of Experimental Equipment and Materials:

Equipment included a DC pulse power supply for MAO, FE-SEM (HITACHI S-4800), EDS (Bruker), confocal laser scanning microscope, micro-CT, and various kits for assays (e.g., CCK-8, EdU, LIVE/DEAD). Materials included sodium hexametaphosphate, sodium hydroxide, ethylene diamine tetraacetic acid, sodium citrate, Zn gluconate, Sr gluconate, fetal bovine serum, α-MEM, and others.

4：Experimental Procedures and Operational Workflow:

Samples were prepared via MAO, characterized using SEM and EDS, and subjected to protein adsorption, cytotoxicity, cell adhesion, proliferation, differentiation, antibacterial tests, and in vivo implantation in rabbits with subsequent X-ray, micro-CT, and histological analyses.

5：Data Analysis Methods:

Data were analyzed using statistical techniques, with qRT-PCR for gene expression, Western blot for protein analysis, and image analysis for cell and bacterial counts.