研究目的
Designing a novel fluorescent photonic switch for selective detection of H2PO4? and CN? ions using an Al3+-morpholine anthracene chelate, and demonstrating its applications in photonic logic circuits and biological imaging.
研究成果
The Al3+-morpholine anthracene chelate serves as an effective dual photonic switch for H2PO4? and CN? ions, with high selectivity and sensitivity. It enables combinational logic circuits and image-guided tracking in biological systems, demonstrating potential for bio-optoelectronic applications.
研究不足
The study is limited to in vitro conditions in mixed aqueous media (DMSO-H2O); potential cytotoxicity and low photo-stability in physiological conditions may restrict in vivo applications; the masking effect of CN? ions complicates simultaneous detection with H2PO4?.
1：Experimental Design and Method Selection:
The study involved synthesizing probe 1 and its Al3+ chelate in situ, using UV-Vis, fluorescence, NMR, and FT-IR spectroscopy for characterization and sensing. DFT calculations supported the structural analysis.
2：Sample Selection and Data Sources:
Samples included probe 1, Al(ClO4)3, and various anions (e.g., H2PO4?, CN?) in DMSO-H2O (1:1 v/v) at pH
3：3 (HEPES buffer). Candida albicans cells were used for biological applications. List of Experimental Equipment and Materials:
Instruments included FT-IR spectrometer (ATR method), NMR spectrometer (1D and 2D), UV-Vis spectrophotometer, fluorescence spectrophotometer, FAB mass spectrometer, and confocal laser fluorescence microscope. Materials included DMSO, H2O, HEPES buffer, Al(ClO4)3, tetrabutylammonium salts of anions, and probe
4：Experimental Procedures and Operational Workflow:
Synthesis of probe 1 via SN2 reaction; in situ preparation of 1(cid:129)Al3+ complex; titration with anions; NMR and fluorescence measurements; DFT calculations; biological imaging in C. albicans cells.
5：Data Analysis Methods:
Association constants calculated from fluorescence titration; LODs determined; Job's plot for stoichiometry; NMR and mass spectrometry for structural validation; DFT for energy minimization.
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