研究目的
To design and use a microfluidic system combined with terahertz technique for cell detection, aiming to differentiate various kinds of materials and potentially distinguish cancer cells from normal cells.
研究成果
The microfluidic device successfully lowers terahertz absorption from aqueous solutions and demonstrates capability to differentiate materials. Cell trapping results show concentration at electrode edges due to positive DEP, but improvements are needed in buffer composition and electrical parameters. Future work involves injecting different cells and media to achieve differentiation of tumor cells using terahertz signal analysis.
研究不足
The microfluidic device has room for improvement; not all HCT-8 cells are effectively trapped due to issues like cell sticking, suboptimal DEP buffer mixture, and possibly inappropriate frequency/voltage settings from the function generator; terahertz signals become noisy above 1.5 THz, limiting analysis range.
1：Experimental Design and Method Selection:
The study involves designing a microfluidic device to minimize water absorption of terahertz waves, using dielectrophoresis (DEP) for cell trapping, and employing a reflective terahertz-time domain spectrometer (THz-TDS) for signal detection and analysis via fast Fourier transform (FFT).
2：Sample Selection and Data Sources:
HCT-8 cells (13-26 μm in diameter) mixed with DEP buffer (10 mM HEPES, 236 mM sucrose, 59 mM glucose) are used, with experiments conducted in dry air and water environments.
3：List of Experimental Equipment and Materials:
Includes ITO glass, photoresist (AZ5214), developer (AZ400K), ITO etching solution, SU8-3050 microfluidic mold, PDMS, oxygen plasma bonding equipment, syringe pump, function generator (for AC at 1 MHz, 10 Vpp), photoconductive antenna, metal plate, and Polyscanner-FL7 THz-TDS system.
4：Experimental Procedures and Operational Workflow:
Fabricate microfluidic device with ITO glass substrate and PDMS microchannel using photolithography and bonding; inject cell solution into microchannel using syringe pump at 90 μm/s; apply DEP force via electrodes to trap cells; position device under THz-TDS; detect terahertz signals at eight positions with scan average=4 and step=0.2 mm; transform signals to frequency domain using FFT for comparison.
5：2 mm; transform signals to frequency domain using FFT for comparison.
Data Analysis Methods:
5. Data Analysis Methods: Compare frequency domain signals from different positions to identify material characteristics, focusing on differences between air and water environments.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
