研究目的
To study the stability of silver-based SERS substrates under various physical and chemical loads, including temperature, humidity, and (bio)environmental conditions, to assess their suitability for storage, transportation, and utilization.
研究成果
Silver-based SERS substrates show varying stability under different conditions: mild degradation with separate temperature or humidity treatments, significant loss of efficiency and reproducibility under combined temperature/humidity or BSA solution treatments, and preserved reproducibility but reduced efficiency in physiological solutions. These findings help predict risks in transportation, storage, and use of such substrates.
研究不足
The study focuses on silver-based substrates and may not generalize to other materials. The treatments simulate specific environmental conditions, and real-world scenarios could involve additional factors. The reproducibility is assessed under controlled lab conditions, which might differ in practical applications.
1：Experimental Design and Method Selection:
The study involved systematic testing of SERS substrates under different environmental conditions to evaluate stability. Methods included physical treatments (temperature and humidity cycling) and chemical treatments (immersion in physiological and BSA solutions).
2：Sample Selection and Data Sources:
SERS substrates were prepared as silver-coated periodical gratings on polymer films. Samples were subjected to treatments for various time intervals (e.g., 1 h to 3 months).
3：List of Experimental Equipment and Materials:
Equipment included a climatic test chamber (V?TSCH VC37018), XPS spectrometer (Omicron Nanotechnology ESCAProbeP), SEM (LYRA3 GMU, Tescan), Raman spectrometer (Advantage NIR, DeltaNu), and materials such as Su-8 polymer, silver target, R6 G analyte, and various solutions.
4：Experimental Procedures and Operational Workflow:
Substrates were prepared by spin-coating polymer films, patterning with a KrF excimer laser, and sputtering silver. Physical treatments involved cyclic changes in temperature and humidity in the climatic chamber. Chemical treatments involved immersion in solutions for specified times. After treatments, samples were analyzed using XPS, SEM, and Raman spectroscopy to measure SERS efficiency and reproducibility.
5：Data Analysis Methods:
SERS intensity and reproducibility were measured using Raman spectroscopy with R6 G as the analyte. Data were analyzed based on changes in peak intensity and convergence from multiple measurements.
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