研究目的
To solve the error caused by maladjustment of optical path length in photometric detection by proposing a round microfluidic chip for accurate concentration detection.
研究成果
The proposed round microfluidic chip effectively compensates for errors caused by fixed optical path length in photometric detection. With optimized parameters, it achieves a compensation ratio of up to 14.22% for TNBA solution, demonstrating high accuracy and acceptable reproducibility. This method provides a foundation for developing high-precision microfluidic detection equipment.
研究不足
The detected concentration range is limited to between 0.002 mol/L and 0.05 mol/L in the three-layer microfluidic chip. The method requires optimization of injection velocity and concentration, which may increase experiment time. It is specific to microfluidic applications and may not be directly applicable to macroscopic systems.
1：Experimental Design and Method Selection:
The study uses a round microfluidic chip designed to create a continuous gradient distribution of concentration. Theoretical analysis based on Lambert-Beer law is employed to model absorbance and error. The method involves dynamic searching for optimum absorbance and calculating concentration using arc length relationships.
2：Sample Selection and Data Sources:
TNBA solution with concentrations from 1×10^{-3} to 0.01 mol/L is used as the detection object, simulating pesticide residue detection.
3：01 mol/L is used as the detection object, simulating pesticide residue detection. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Includes a spectrometer (HR2000+ES, Ocean Optics), light source (HL-2000-FUSA, Ocean Optics), syringe pump (LSO04-1AZ, LongerPump), optical fiber lines (VIS-NIR, Ocean Optics), fiber bracket, stepping motor (57BYG250B, TELESKY), microfluidic chip, and computer. The microfluidic chip has specific dimensions as detailed in the paper.
4：Experimental Procedures and Operational Workflow:
Solutions and water are injected into the chip via syringes. The photoelectric detection system is powered, with the stepping motor rotating the chip. Light from the source passes through the concentration gradient area, and absorbance is measured by the spectrometer. Data is analyzed to find optimum absorbance and calculate concentration using derived equations.
5：Data Analysis Methods:
Mixing index is calculated to assess uniformity. Compensation ratio is computed to evaluate error reduction. Statistical analysis includes relative standard deviation for reproducibility.
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spectrometer
HR2000+ES
Ocean Optics
Used to measure absorbance in the photoelectric detection system.
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light source
HL-2000-FUSA
Ocean Optics
Provides light for photometric detection.
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optical fiber lines
VIS-NIR
Ocean Optics
Transmit light between the source, chip, and spectrometer.
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syringe pump
LSO04-1AZ
LongerPump
Used to inject solutions into the microfluidic chip.
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stepping motor
57BYG250B
TELESKY
Rotates the microfluidic chip for dynamic detection.
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