1：Experimental Design and Method Selection:

The study involves the use of a radio-fluorogenic gel based on γ-ray-polymerized tertiary-butyl acrylate (TBA) with a fluorogenic compound like maleimido-pyrene (MPy). The method relies on radiation-induced copolymerization to convert non-fluorescent MPy into fluorescent SPy, with fluorescence intensity proportional to local radiation dose.

2：Sample Selection and Data Sources:

Gels are prepared using de-aerated TBA and MPy solutions, irradiated with various radiation sources (e.g., γ-rays, electrons, X-rays, protons) to induce polymerization and fluorescence.

3：List of Experimental Equipment and Materials:

Includes a nitrogen-flushed glove box, 60Co γ-ray source (GC200 from Atomic Energy of Canada), Van de Graaff electron accelerator, X-ray sources (e.g., Philips MCN 321), proton cyclotron, cameras (RICOH Caplio RX and GX200), UV lamps, and software like ImageJ for data analysis. Materials include TBA (Sigma-Aldrich #327182), MPy (Sigma-Aldrich P7908), and various cells for gel containment.

4：Experimental Procedures and Operational Workflow:

Gel preparation involves radiation-induced polymerization of TBA to ~15% conversion, removal of residual monomer, replacement with MPy solution, and gel regeneration. Imaging is done using UV light excitation and digital cameras to capture fluorescence, with data processed in ImageJ.

5：Data Analysis Methods:

Fluorescence images are analyzed for intensity profiles and spatial resolution using pixel scanning in ImageJ, with calibration against known dose rates and comparisons to theoretical models.