研究目的
The main goal of the present investigation was to the synthesis of bioactive TiO2 nanoparticle-incorporated poly(methylmethacrylate)/polyetheretherketone nanocomposite for enhanced antibacterial and biocompatibility characteristics with suitable properties for manufacturing 3D printed stereolitographic dental prosthesis.
研究成果
The bioactive copolymeric nanocomposite with carboxylic acid-functionalized nano-TiO2 was successfully prepared through an emulsion polymerization process, and it has been employed to manufacturing a complete stereolithographic dental prosthesis. The spectral analyses are clearly established the successful functionalization of TiO2 and also exhibited the monodispersed nature of particle structure. The microscopic investigations of nanocomposites visualized that particles are formed to spherically shaped with less degree of agglomeration under PEEK/PMMA copolymer matrixes. The bioactive functionalized TiO2 nanoparticles are highly dispersed in the prepared dental composite materials, which has been excellently influenced bactericidal activity on the gram-positive and gram-negative bacterial pathogens and also altered the cytocompatibility properties with dental pulp cells. This facile synthesized nanocomposite was effectively employed for complete denture manufacturing with stereolitographic technique.
研究不足
The paper does not explicitly mention specific limitations, but potential areas could include the scalability of the synthesis process, long-term stability of the nanocomposite, and clinical applicability beyond laboratory settings.
1：Experimental Design and Method Selection:
The study involved synthesizing carboxylic acid-functionalized TiO2 nanoparticles and incorporating them into a PMMA/PEEK copolymer matrix using emulsion polymerization to create a nanocomposite for dental applications. Methods included functionalization with acrylic acid, polymerization, and various characterization techniques.
2：Sample Selection and Data Sources:
Nano-TiO2 particles were purchased and functionalized. Bacterial pathogens (Escherichia coli and Staphylococcus aureus) and human dental pulp stem cells (hDPSCs) were used for antibacterial and cytocompatibility tests.
3：List of Experimental Equipment and Materials:
Equipment included ultrasonic processor, centrifuge, vacuum oven, jacketed reactor with stirrer and reflux condenser, XRD (PANalytical’s X’Pert PRO MRD diffractometer), FTIR (SHIMADZU), SEM (Carl Zeiss AURIGA), TEM (Hitachi H8100), CLSM (Nikon A1R), microplate reader. Materials included TiO2 nanoparticles, acrylic acid, hexane, ethyl alcohol, PEEK, MMA monomer, potassium persulphate, nutrient broth, FITC stain, collagenase type I, dispase, DMEM, fetal calf serum, penicillin, streptomycin, MTT assay kit.
4：Experimental Procedures and Operational Workflow:
Functionalization of TiO2 with acrylic acid under sonication and agitation, followed by centrifugation and drying. Synthesis of nanocomposite by dispersing functionalized TiO2 in PEEK solution, adding to MMA, and polymerizing with initiator at 70°C. Characterization using XRD, FTIR, SEM, TEM, EDX. Antibacterial analysis via agar diffusion and solution suspension methods, CLSM imaging. Cytocompatibility analysis using hDPSCs culture and MTT assay.
5：Data Analysis Methods:
Data from spectroscopic and microscopic techniques were analyzed to confirm structural and morphological properties. Antibacterial efficiency was calculated as % survival = B/A × 100. Cell viability was measured using absorbance in MTT assay.
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