研究目的
To study which orientation (equatorial region or blunt end) is better for freshness prediction of white-shelled eggs using visible near infrared spectroscopy, and to develop models for predicting Haugh unit, yolk index, and albumen pH.
研究成果
The equatorial region provides better prediction accuracy for egg freshness parameters (Haugh unit, yolk index, albumen pH) compared to the blunt end, with correlation coefficients of 0.881, 0.855, and 0.888, respectively. This suggests that the equatorial region is more suitable for nondestructive freshness assessment using VIS-NIR spectroscopy, likely due to more homogeneous eggshell texture and arrangement of internal components. Future research could explore other egg types and improve model robustness.
研究不足
The study is limited to white-shelled eggs and specific storage conditions. Manual errors in spectral measurement and egg properties (e.g., shell thickness variations) may affect results. The models may not generalize to other egg types or colors. The chemical basis for spectral changes is not fully understood.
1：Experimental Design and Method Selection:
The study used visible near infrared (VIS-NIR) transmission spectroscopy to acquire spectra from eggs in two orientations (equatorial region and blunt end). A partial least squares regression (PLSR) model was developed to predict freshness parameters (Haugh unit, yolk index, albumen pH). Pretreatment methods included Savitzky-Golay smoothing, multiplicative scatter correction, standard normal variate, first derivative, and second derivative. Full cross-validation was used to select principal components and avoid overfitting.
2：Sample Selection and Data Sources:
Fresh white-shelled commercial eggs from 41-week-old White Leghorn hens were collected. 91 eggs were measured after storage for 0, 1, 3, 5, 7, 8, 10, 12, 13, 16, 18, 19, and 21 days at 30°C and 60% relative humidity to ensure variability in freshness parameters.
3：List of Experimental Equipment and Materials:
Halogen lamp (100 W), USB 2000+ spectrometer (Ocean Optics Inc.), Teflon blocks (Jiangxingsifu), electronic balance (FA 2004B, Shanghai Yoke Instrument Co.), height micrometer, digital caliper, pH-meter (FE28, METTLER TOLEDO), dark box, cooling fan, fiber probe, optical fiber, computer with MATLAB software (2014a, Mathworks Inc.).
4：Experimental Procedures and Operational Workflow:
Spectra were acquired in a dark box to avoid external light. The light source was preheated for 30 minutes. Spectra were measured in equatorial region and blunt end with specific Teflon block thicknesses (8 mm and 14 mm) and integration times (23 ms and 46 ms). Three replicate measurements were averaged. After spectral measurement, destructive measurements of Haugh unit, yolk index, and albumen pH were performed using standard methods.
5：Data Analysis Methods:
Spectral data were preprocessed using MATLAB. PLSR was applied to build models, with performance evaluated by correlation coefficients (Rc, Rp) and root mean square errors (RMSEC, RMSEP).
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
