研究目的
To develop a hydrophilic TADF emitter for time-resolved luminescence imaging in living cells by addressing the challenge of oxygen quenching and enabling specific mitochondrial targeting through aggregation.
研究成果
The study successfully developed a hydrophilic TADF emitter with aggregation-induced delayed fluorescence enhancement, enabling effective time-resolved and two-photon luminescence imaging of mitochondria in living cells, reducing background signals and improving specificity.
研究不足
The hydrophilic nature of NID-TPP may limit aggregation in non-targeted cellular environments, and the sensitivity to oxygen in non-aggregated states could affect performance. Further optimization for broader biological applications is needed.
1：Experimental Design and Method Selection:
The study designed and synthesized a hydrophilic TADF luminophore (NID-TPP) with a mitochondria-targeting TPP+ group. Methods included density functional theory simulations, photoluminescence spectroscopy, and cell imaging techniques.
2：Sample Selection and Data Sources:
Used HeLa cells for biological imaging, and chemical samples like NID and NID-TPP synthesized in the lab.
3：List of Experimental Equipment and Materials:
Included spectrometers, microscopes, and chemicals such as tetrahydrofuran, dimethyl sulfoxide, and sodium tetraphenylborate.
4：Experimental Procedures and Operational Workflow:
Synthesized NID-TPP, measured its photophysical properties in various solvents and aggregated states, performed cell culture and staining, and conducted time-resolved and two-photon imaging.
5：Data Analysis Methods:
Analyzed emission spectra, lifetime decays, and colocalization coefficients using appropriate software and statistical methods.
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