1：Experimental Design and Method Selection:

The study involved synthesizing magnetic graphene oxide (mGO) via a solvothermal reaction and functionalizing it with chitosan and sodium alginate using layer-by-layer (LbL) self-assembly to improve dispersibility and stability. Methods included TEM, AFM, zeta potential analysis, FT-IR, XRD, stability tests, protein adsorption assays, drug loading and release studies, cytotoxicity assays, cellular uptake imaging, and photothermal property assessments.

2：Sample Selection and Data Sources:

Samples included GO, mGO, mGO-CS, mGO-CS/SA, and DOX-loaded variants. Human lung cancer cell line A549 was used for in vitro studies. Data were sourced from experimental measurements and characterizations.

3：List of Experimental Equipment and Materials:

Equipment included TEM (JEOL JEM 2010), AFM (Bruker Multimode 8), zeta potential analyzer (NanoBrook 90 Plus PALS), FT-IR spectrometer (Nicolet Nexus), XRD (Shimadzu XRD-6000), UV spectrophotometer, fluorescence spectrophotometer (Varian Inc.), vibrating sample magnetometer, CLSM, and laser irradiation setup (808 nm, 1 W/cm2). Materials included chitosan, sodium alginate, DOX, FeCl3·6H2O, ethylene glycol, diethylene glycol, sodium acrylate, sodium acetate, BSA, and cell culture reagents.

4：Experimental Procedures and Operational Workflow:

mGO was synthesized by mixing GO with ethylene glycol, diethylene glycol, sodium acrylate, sodium acetate, and FeCl3·6H2O, followed by solvothermal reaction. mGO-CS/SA was prepared by dispersing mGO in chitosan solution, filtering, washing, then adding sodium alginate solution, stirring, sonicating, filtering, and washing. DOX loading was done by mixing with DOX solution, stirring, and centrifuging. Stability tests involved incubating samples in water, PBS, and cell culture media. Protein adsorption was measured with BSA. Drug release was studied using dialysis bags at pH 7.4 and 5.0. Cytotoxicity was assessed via MTT assay. Cellular uptake was imaged with CLSM using FITC-labeled samples. Photothermal properties were evaluated by laser irradiation and temperature measurement.

5：4 and Cytotoxicity was assessed via MTT assay. Cellular uptake was imaged with CLSM using FITC-labeled samples. Photothermal properties were evaluated by laser irradiation and temperature measurement. Data Analysis Methods:

5. Data Analysis Methods: Data were analyzed using statistical methods (mean ± S.D. for triplicates), UV-Vis and fluorescence spectroscopy for quantification, and imaging techniques for morphological and cellular assessments.