1：Experimental Design and Method Selection:

The study involved synthesizing Fe3O4@PB nanoparticles and evaluating their photothermal and antibacterial properties under solar irradiation. The design rationale was to combine the photothermal effect of Prussian blue with the magnetic properties of Fe3O4 for recyclable sterilization.

2：Sample Selection and Data Sources:

Escherichia coli and Staphylococcus aureus bacteria were used as models, cultured in LB medium. Contaminated tap water from Songhua River was also tested.

3：List of Experimental Equipment and Materials:

Materials included potassium ferrocyanide trihydrate, iron(III) chloride, urea, ethylene glycol, and others from Aladdin. Equipment included SEM (Hitachi Limited), FT-IR spectrometer (Nicolet 6700, Thermo Scientific), XRD diffractometer (Bruker D8 advance), SQUID (MPMSXL-7 Tesla, Quantum Design), UV-vis-NIR spectrophotometer (Evolution 300, Thermo Scientific), solar simulator, laser (808 nm, Beijing Kaipulin Optoelectronic Technology Co.), thermocouple microprobe (STPC-510P, Xiamen Baidewo Technology Co.), and autoclave.

4：Experimental Procedures and Operational Workflow:

Synthesis of Fe3O4 cores via polyol method, coating with PB, characterization (SEM, XRD, FT-IR, magnetic properties, optical absorption), photothermal effect measurements, bacterial culture and preparation, photothermal sterilization tests, SEM for morphological changes, recyclability tests with magnetic separation, and tests in contaminated tap water.

5：Data Analysis Methods:

Colony counting for bacterial viability, temperature monitoring, UV-vis-NIR spectroscopy for absorption, and statistical analysis of results.