研究目的
To assess soil carbon stocks and humification in different agro-ecologies on the eastern foot slopes of Mount Kenya, determine differences related to soil texture, altitude, climate, and land use, and evaluate spectroscopic techniques (LIFS and NIRS) for carbon quantification and humification assessment.
研究成果
The study demonstrated significant variations in soil carbon stocks and humification across different land uses and agro-ecologies. NIRS and LIFS were effective for carbon quantification and humification assessment, showing potential for portable, low-cost field applications. These methods can support sustainable soil management and climate-smart agriculture practices.
研究不足
The study is limited to a specific region (Embu, Kenya) and may not be generalizable to other areas. The prototype LIFS equipment was used in the lab, not in situ, and NIRS had higher detection limits for low carbon samples. Soil heterogeneity and the use of a fixed depth (0-30 cm) could affect results.
1：Experimental Design and Method Selection:
The study involved comparing agricultural systems (tea, coffee, maize-based intercropping) with natural vegetation along a transect. Spectroscopic methods (LIFS and NIRS) were used alongside conventional carbon analysis (CHN) to assess carbon stocks and humification.
2：Sample Selection and Data Sources:
Soil samples were collected in triplicates from five depth layers (0-30 cm) in three sites in Embu, Kenya, covering different land uses and agro-ecologies. Samples were air-dried, sieved, and transported for analysis.
3：List of Experimental Equipment and Materials:
Equipment included a Perkin-Elmer 2400 CHNS/O analyzer for carbon analysis, an Embrapa-built LIFS apparatus with a 405 nm laser, and a Perkin-Elmer Spectrum 100N NIRS spectrometer with ATR accessory. Materials included soil samples, aluminum rings for bulk density, sieves (2 mm and 0.25 mm), and software (Quant+ for NIRS).
4：25 mm), and software (Quant+ for NIRS).
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Soil sampling involved excavating pits for bulk density measurement. Samples were processed by drying, crushing, sieving, and analyzing for total carbon using CHN, humification using LIFS, and carbon quantification using NIRS. Statistical analysis (ANOVA, Tukey test) was performed.
5：Data Analysis Methods:
Data were analyzed using ANOVA for significant differences, PLS regression for NIRS calibration, and correlation coefficients for method validation. Carbon stocks were calculated using bulk density and carbon content.
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