研究目的
To investigate the effects of Ca2+ and Mg2+ on the solar photodegradation of oxytetracycline (OTC) in brackish aquaculture water, including kinetics, quantum yields, and photoproduct formation.
研究成果
Ca2+ enhances OTC photodegradation kinetics, while Mg2+ inhibits this effect and influences photoproduct formation. Similar binding patterns for Ca2+ and Mg2+ are proposed, with differences in photolysis and fluorescence quantum yields. Mg2+ induces the formation of new photoproducts and inhibits others, indicating different degradation mechanisms.
研究不足
The study is limited to specific conditions (pH 7.3, certain cation concentrations) relevant to brackish aquaculture water, and may not be fully extrapolatable to other environmental matrices. The use of simulated sunlight might not perfectly replicate natural conditions.
1：Experimental Design and Method Selection:
The study used UV-vis and fluorescence spectroscopy to investigate complexation patterns, and photodegradation experiments under simulated sunlight to assess kinetics and photoproducts.
2：Sample Selection and Data Sources:
OTC solutions were prepared in phosphate buffer with NaCl, Ca2+, and/or Mg2+ at concentrations relevant to brackish water. Real aquaculture water samples were also used.
3：List of Experimental Equipment and Materials:
Equipment includes a T90+ UV/VIS Spectrophotometer, FluoroMax-4 spectrofluorometer, Solarbox 1500 sunlight simulator, and HPLC-DAD with fluorescence detector. Materials include OTC hydrochloride, NaCl, CaCl
4：2H2O, MgCl6H2O, phosphate buffer, and formic acid. Experimental Procedures and Operational Workflow:
UV-vis and fluorescence spectra were acquired for various OTC solutions. Photodegradation experiments involved irradiating solutions in quartz tubes using the sunlight simulator, with samples taken at intervals for HPLC analysis. Dark controls were maintained.
5：Data Analysis Methods:
Kinetic data were fitted to first-order kinetics using GraphPad Prism 6. Spectroscopic data were analyzed to infer complexation patterns and quantum yields.
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