研究目的
To study the kinetics of delayed fluorescence and phosphorescence of xanthene dyes in mouse tissues for continuous monitoring of oxygen concentration in vivo and in vitro, and to propose a method for determining oxygen recovery time during photodynamic processes.
研究成果
The study demonstrates that continuous in vivo monitoring of oxygen concentration in tissues is feasible using the kinetics of delayed fluorescence and phosphorescence of xanthene dyes. The annihilation component of delayed fluorescence is highly sensitive to oxygen changes, making it useful for real-time monitoring in photodynamic therapy. Future work could optimize dye stability and extend applications to other tissues.
研究不足
The method relies on exogenous fluorophores, which may photochemically burn out over time. It is sensitive to the specific dyes used and requires controlled experimental conditions. The approximation in kinetic equations may not hold for all tissue types or oxygen concentrations.
1：Experimental Design and Method Selection:
The study involved in vivo and in vitro experiments using pulse-periodic excitation of xanthene dyes to monitor oxygen concentration via delayed fluorescence and phosphorescence kinetics. Theoretical models included kinetic equations for delayed fluorescence intensity.
2：Sample Selection and Data Sources:
Malignant tumors and normal epithelial and muscle tissues from BYRB mice were used. Tissues were stained with xanthene dyes (erythrosine, eosin, rose bengal) by immersion in aqueous solutions.
3：List of Experimental Equipment and Materials:
Equipment included a solid-state YAG:Nd3+ laser for excitation, an FEU-84 photoelectric multiplier with a control electrode, an MDR-41 monochromator, and a chamber with nitrogen gas for oxygen control. Materials included xanthene dyes and mouse tissues.
4：Experimental Procedures and Operational Workflow:
Tissues were stained and placed in a chamber. Excitation was done with laser pulses at 532 nm, and luminescence was recorded at specific wavelengths. Oxygen concentration was varied by nitrogen gas or oxygen mask in vivo. Kinetic curves were measured after pulse series.
5：Data Analysis Methods:
Data analysis involved measuring areas under kinetic curves for delayed fluorescence and phosphorescence, using normalization and comparison to initial pulses to assess oxygen concentration changes.
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