研究目的
To study the time dependence of charge accumulation in flowing albumin solutions at physiological temperatures and its dependence on concentration, for application in biosensor systems.
研究成果
Charge accumulation in flowing albumin solutions exhibits nonlinear behavior with sharp increases at low concentrations (10?15 M) but not at higher concentrations (10?4 M), observed at both 35°C and 38°C. This effect must be considered in developing analytical flow systems for biomolecule detection to avoid measurement inaccuracies and to enhance detection efficiency.
研究不足
The study is limited to specific temperatures (35°C and 38°C) and concentrations (10?15 M and 10?4 M BSA). The mechanism of charge generation is not fully understood, and the experiments rely on a specific flow system, which may not generalize to other setups. Potential optimizations include exploring a wider range of temperatures and concentrations.
1：Experimental Design and Method Selection:
The study used a flow-through system based on an AFM fishing injector to monitor charge generation in a measuring cell. Electric charge measurements were performed using an electrometer. The rationale was to observe electrokinetic phenomena in protein solutions under controlled conditions.
2：Sample Selection and Data Sources:
Samples included deionized water, 10?15 M BSA solution, and 10?4 M BSA solution. BSA was fatty acid-free from Sigma. Solutions were prepared by sequential dilutions.
3：List of Experimental Equipment and Materials:
Equipment included a peristaltic pump (Ismatech, IDEX), silicone tubing (length 40 cm, i.d. 2.0 mm), disposable pipet tips (for 1–10 μL pipet, i.d. 0.4 mm), a measuring cell (stainless steel inner cylinder), an electrometer (Keithley 617), a grounding electrode, and a Millipore Simplicity UV system for deionized water. Materials included BSA (Sigma cat. No. A6003) and polypropylene tubes.
4：0 mm), disposable pipet tips (for 1–10 μL pipet, i.d. 4 mm), a measuring cell (stainless steel inner cylinder), an electrometer (Keithley 617), a grounding electrode, and a Millipore Simplicity UV system for deionized water. Materials included BSA (Sigma cat. No. A6003) and polypropylene tubes.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The system was filled and washed with the liquid before experiments. The pump delivered the liquid dropwise into the measuring cell. Charge was measured every 15 s for 7 minutes using the electrometer. Experiments were conducted at 35°C and 38°C, with tubing and tips replaced after each series.
5：Data Analysis Methods:
Data were presented as charge value over baseline (Δq) and analyzed for linear and abrupt changes in charge accumulation over time.
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