研究目的
To evaluate the effects of platelet aggregate formation on light transmission aggregometry results.
研究成果
Platelet aggregate formation in citrated blood samples reduces light transmission aggregometry maximum aggregation percentage, likely due to the removal of large activated platelets during the measurement process. This interference affects the accuracy of assessing platelet hyperactivity, so confirming the absence of platelet aggregates in samples is necessary for reliable results.
研究不足
The study could not evaluate PRP after platelet aggregate formation using all concentrations of agonists, and different centrifugation speeds in the PRP separation process were not tried, although a slow centrifugation speed was used to minimize removal of active platelets.
1：Experimental Design and Method Selection:
The study used a hematology analyzer to intentionally induce platelet aggregate formation in citrated blood samples and compared light transmission aggregometry results before and after this formation. Fully automated light transmission aggregometry was employed with agonists like adenosine diphosphate and collagen.
2：Sample Selection and Data Sources:
19 citrated blood samples were obtained from 6 healthy volunteers and 13 patients with various cerebrovascular conditions. Samples were selected based on the detection of platelet aggregates on a scattergram of a hematology analyzer.
3：List of Experimental Equipment and Materials:
Hematology analyzer (CELL-DYN Sapphire Hematology System; Abbott Diagnosis), fully automated LTA machine (CS-2000i; Sysmex Corporation), agonists (Revohem adenosine diphosphate and Revohem Collagen from Sysmex Corporation), EDTA-2K-containing tubes, sodium citrate-containing tubes, and a 21G needle for blood collection.
4：Experimental Procedures and Operational Workflow:
Blood was drawn and analyzed using the hematology analyzer to induce platelet aggregate formation. PRP and PPP were prepared by centrifugation. LTA was performed with various agonist concentrations, and platelet count and mean platelet volume were measured before and after platelet aggregate formation.
5：Data Analysis Methods:
Statistical analyses were conducted using JMP 10.0.2 software, including Mann-Whitney U test, Student's t-test, and Wilcoxon signed-rank test, with significance set at P < .05.
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CELL-DYN Sapphire Hematology System
Sapphire
Abbott Diagnosis
Used for hematology analysis to detect platelet aggregates in blood samples.
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CS-2000i
CS-2000i
Sysmex Corporation
Fully automated light transmission aggregometry machine for measuring platelet aggregation.
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Revohem adenosine diphosphate
Sysmex Corporation
Agonist used in light transmission aggregometry to induce platelet aggregation.
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Revohem Collagen
Sysmex Corporation
Agonist used in light transmission aggregometry to induce platelet aggregation.
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EDTA-2K-containing tube
Used for blood collection and analysis to prevent coagulation.
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Sodium citrate-containing tube
Used for blood collection with sodium citrate as an anticoagulant.
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21G needle
21G
Used for drawing blood from the antecubital vein.
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JMP
10.0.2
SAS Institute Inc.
Software used for statistical analysis of the data.
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