研究目的
To investigate the application of a carbon arc lamp on wound healing in a rat cutaneous full-thickness wound model and the corresponding mechanisms.
研究成果
Carbon arc lamp irradiation significantly accelerates wound healing and improves healing quality by stimulating cell proliferation, as evidenced by increased wound healing rate, epithelial edge length, collagen content, microvessel density, and BrdU-labeled cells. This suggests potential for clinical application, but further studies on parameters and comparisons are needed.
研究不足
Lack of research on the influence of optical parameters (e.g., power density, spot area, irradiation time) on therapeutic effect, and no comparison with other light sources like laser or LED in terms of mechanism and efficacy.
1：Experimental Design and Method Selection:
A controlled experiment was designed with two groups (treated and control) to assess the effects of carbon arc lamp irradiation on wound healing in rats. Methods included histological analyses, BrdU immunohistochemical staining, and statistical comparisons.
2：Sample Selection and Data Sources:
Thirty male Wistar rats (9-10 weeks old, 250-300 g) were used, randomly assigned to treated (irradiated) and control (sham-irradiated) groups. Data were collected from wound measurements and tissue samples at days 7, 14, and 21 post-wounding.
3：List of Experimental Equipment and Materials:
Carbon arc lamp (YW-0828A type with red medical carbon), thermopile power meter, pentobarbital sodium solution, Er iodine disinfectant, BrdU (B5002-250MG), anti-BrdU antibody (B2531-.2ML), streptavidin-biotin-peroxidase kits (Histostain-SP Kits, SPN-9002), ImageJ software, SPSS software, standard laboratory equipment for histology (e.g., microscope, formalin, H&E staining, Masson staining).
4：1-.2ML), streptavidin-biotin-peroxidase kits (Histostain-SP Kits, SPN-9002), ImageJ software, SPSS software, standard laboratory equipment for histology (e.g., microscope, formalin, H&E staining, Masson staining). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Rats were anesthetized, dorsal hair removed, and a 2x2 cm full-thickness wound created. Irradiation was applied daily at 20 cm distance with parameters: wavelength 620-740 nm, irradiance 45 mW/cm2, duration 1200 sec, energy density 54 J/cm2. Sham irradiation for controls. Rats euthanized at specified days; wounds photographed and analyzed for area healing rate. Tissue samples fixed, sectioned, stained (H&E, Masson, BrdU immunohistochemistry), and examined microscopically.
5：Sham irradiation for controls. Rats euthanized at specified days; wounds photographed and analyzed for area healing rate. Tissue samples fixed, sectioned, stained (H&E, Masson, BrdU immunohistochemistry), and examined microscopically. Data Analysis Methods:
5. Data Analysis Methods: Wound healing rate calculated using ImageJ. Statistical analysis performed with SPSS using independent-samples t-test; p<0.05 considered significant. Data presented as mean ± standard deviation.
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BrdU
B5002-250MG
Sigma Chemical Co.
Used for labeling proliferative cells in immunohistochemical staining to assess cell proliferation.
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anti-BrdU antibody
B2531-.2ML
Sigma Chemical Co.
Used in immunohistochemical staining to detect BrdU-labeled cells.
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carbon arc lamp
YW-0828A
GuangDong Ewan Biomedicine Technology Co. Ltd.
Used for irradiating wounds to promote healing through photobiomodulation.
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streptavidin-biotin-peroxidase kits
SPN-9002
Zymed Laboratories
Used for immunostaining in BrdU immunohistochemical procedures.
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ImageJ software
National Institutes of Health
Used for analyzing wound size and histological images to measure healing rates and other parameters.
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SPSS software
Used for statistical analysis of experimental data.
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