研究目的
To develop predictive equations for post-mortem interval using spectrophotometric values of post-mortem lividity and analyze the relationship between post-mortem lividity, control skin, and blood colors.
研究成果
The study successfully developed predictive equations for post-mortem lividity color and PMI using spectrophotometric data. The equation for PMI without blood color values had an RMSE of 10.29 hours, indicating reasonable accuracy. Blood color contributed to post-mortem lividity but was not a major factor. Further research is needed to understand additional influencing factors and improve equation accuracy.
研究不足
The study excluded cadavers with PMI >72 hours, charred bodies, or putrefaction, limiting generalizability. Not all cases had blood color measurements from all sites (e.g., femoral vein n=21), reducing sample size for some analyses. The equations may have overfitting issues, and factors not considered could affect accuracy. Blood color was not strongly correlated with post-mortem lividity, suggesting other unaccounted factors.
1:Experimental Design and Method Selection:
The study used spectrophotometry to objectively measure color values (L*, a*, b*) of post-mortem lividity, control skin, and blood from cadavers. Statistical analyses included Pearson correlation coefficients, linear regression, and stepwise regression to develop predictive equations.
2:Sample Selection and Data Sources:
86 adult cadavers from forensic autopsy cases at Kyushu University between August 2007 and April 2012 were included. Cadavers with PMI >72 hours, charred bodies, or putrefaction were excluded. Data included age, sex, cause of death, PMI, and autopsy findings.
3:List of Experimental Equipment and Materials:
Spectrophotometer (CM-2600d; Konica Minolta, Inc., Tokyo, Japan), plastic cell (CM-A130; Konica Minolta, Inc., Tokyo, Japan) for blood measurement, and statistical software (JMP?, Version 11.2, SAS Institute, Inc.).
4:2, SAS Institute, Inc.).
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Color measurements were taken at multiple skin sites (e.g., upper arms, chest) for post-mortem lividity and control skin. Blood was collected from left heart, right heart, and femoral vein. Measurements were performed using the L*a*b* color system.
5:Data Analysis Methods:
Pearson correlation for relationships, linear regression for PMI relationships, stepwise regression for predictive equations, with significance at p < 0.05. Variance inflation factors >10 were excluded to avoid multicollinearity.
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