研究目的
To develop a simple, low-cost, and instrument-free method for fabricating microfluidic paper-based devices (μPADs) using spray painting and hole punching, and to apply this for colorimetric detection of iron.
研究成果
The spray painting technique provides a cost-effective and simple method for fabricating μPADs, enabling sensitive iron detection with a linear range of 0-0.02 g/L and a low detection limit of 0.00090 g/L. The device shows good selectivity, reproducibility, and acceptable recovery in real samples, suggesting potential for environmental monitoring and in-field use. Future work should explore multi-element detection.
研究不足
The application is limited to single analyte detection (iron in this case), and the method may have constraints in multi-element detection or complex sample matrices. Potential optimizations include extending to other analytes and improving device robustness for field applications.
1：Experimental Design and Method Selection:
The study designed a novel spray painting technique to create hydrophobic barriers on paper for μPADs, using a hole puncher for patterning without specialized instruments. Colorimetry was chosen for detection due to its simplicity and accessibility with cell phone cameras.
2：Sample Selection and Data Sources:
Filter paper (Whatman No. 1) and printer paper were used as substrates. Iron standards and interferents (various cations) were prepared from analytical grade reagents. Tap water samples were used for recovery tests.
3：List of Experimental Equipment and Materials:
Aerosol spray paint (black, 400 mL/can), hand punch (6 mm diameter), cell phone (Redmi Note 4X), photography box (40*40*40 cm with 84 LED lights), vacuum oven, Image-j software, double-sided adhesive tape, and chemicals like FeSO4·7H2O and 1,10-phenanthroline.
4：Experimental Procedures and Operational Workflow:
Fabrication involved spraying printer paper with paint, drying, punching patterns, assembling layers with adhesive tape, pre-treating filter paper dots with reagents, adding sample solutions, reacting for 7 minutes, capturing images with a cell phone in a controlled light box, and analyzing RGB intensities using Image-j. Optimization of parameters like o-phen concentration and reaction time was performed.
5：Data Analysis Methods:
RGB color values were measured using Image-j software, with linear regression for calibration curves, calculation of detection limits, and statistical analysis of reproducibility and selectivity.
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Aerosol spray paint
400 mL/Can, Black
Used to build hydrophobic barriers on paper for microfluidic devices.
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Hand punch
Circle hole with diameter of 6 mm
Used to cut patterns on filter paper and printer paper for device fabrication.
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Cell phone
Redmi Note 4X
China
Used to capture digital photos of the colored products in detection zones for colorimetric analysis.
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Photography box
40*40*40 cm
Shijiazhuang Ruying Film and Television Equipment Sales Co., Ltd.
Provides controlled lighting environment for imaging the sensors to ensure consistent color measurement.
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Image-j software
National Institute of Health
Used to analyze the average color values in RGB from the captured images for quantitative detection.
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Vacuum oven
Used for drying the sprayed paper and reagent-treated paper dots during fabrication.
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Whatman filter paper
No. 1
Whatman International Ltd.
Used as the substrate material for the microfluidic devices, specifically for the detection zones.
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Double-sided adhesive tape
Used to combine the layers of the microfluidic devices together during assembly.
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