研究目的
To determine measurement variability of N1P1 amplitudes and the R1/R2 ratio in normal subjects and hydroxychloroquine users without retinopathy.
研究成果
mfERG measurements exhibit high test-retest variability, which limits their reliability for single-test decisions in hydroxychloroquine screening. Corroborative testing with other modalities like 10-2 visual field and spectral domain optical coherence tomography is recommended before altering therapy.
研究不足
Relatively small numbers of normal subjects and patients taking hydroxychloroquine. mfERG results vary across instruments, laboratories, and testing conditions, making it difficult to generalize to other settings.
1：Experimental Design and Method Selection:
Retrospective, observational study using multifocal electroretinography (mfERG) to assess test-retest variability and short-term variation. The method of Bland and Altman was used to calculate coefficients of repeatability (COR).
2：Sample Selection and Data Sources:
21 normal subjects and 44 patients taking hydroxychloroquine without retinopathy. Subjects were selected from a private multispecialty practice. Only one eye per subject was included to avoid correlated results.
3：List of Experimental Equipment and Materials:
Espion system (Diagnosys LLC, Lowell, MA, USA) with version 6+ software, DTL fiber electrodes, topical anesthetic, and dilation agents. Stimulation pattern with 61 hexagons, luminances of 1,000 cd/m2 and 0 cd/m2, bandpass filter of 10–100 Hz.
4：Experimental Procedures and Operational Workflow:
mfERGs performed following ISCEV guidelines. Eyes dilated and anesthetized; signals processed and first-order kernel response analyzed. Tests done twice in one session for normals and twice within a year for hydroxychloroquine users.
5：Data Analysis Methods:
Normalized measurements analyzed via Bland and Altman method to calculate COR. Statistical analysis included means, standard deviations, and COR values.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
