研究目的
To design and synthesize a peptide-based PET probe for non-invasive imaging of carbonic anhydrase IX (CA IX) positive tumors, addressing the need for specific molecular imaging of hypoxic tumors.
研究成果
18F-CA IX-P1-4-10 is a promising PET tracer for specific imaging of CA IX-expressing tumors, with good synthesis yield and specificity demonstrated in vivo. Future work should focus on improving stability and expanding to other cancer types.
研究不足
The probe showed significant degradation in serum (51% after 3 hours in NBCS), indicating instability in biological environments. The study was preliminary, using a single tumor model (HT29), and further validation in other models and clinical settings is needed.
1：Experimental Design and Method Selection:
The study utilized click chemistry (1,3-dipolar cycloaddition) for F-18 labeling of a peptide probe targeting CA IX, chosen for its high yield and mild conditions.
2：Sample Selection and Data Sources:
The peptide CA IX-P1-4-10-Yne was purchased, and in vivo studies used HT29 tumor xenograft models in mice.
3：List of Experimental Equipment and Materials:
Included HPLC systems (Agilent 1100 with C18 column), microPET/CT scanner (Siemens Inveon), and various chemicals (e.g., sodium L-ascorbate, copper sulfate).
4：Experimental Procedures and Operational Workflow:
Synthesis involved labeling the peptide with [18F]3, purification via HPLC, stability tests in PBS and NBCS, microPET/CT imaging at specific time points, and immunohistochemistry/western blotting for validation.
5：Data Analysis Methods:
Radio-HPLC for purity and stability, microPET/CT data reconstruction using OSEM3D for SUV calculations, and standard biochemical assays for protein expression.
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