研究目的
To demonstrate the use of natural magnetic nanoparticles from magnetotactic bacteria and their bioinspired hybrids as high-contrast photoacoustic, photothermal, and photomechanical agents for single-cell diagnostics and therapy, particularly for triple negative breast cancer cells and circulating tumor cells in vivo.
研究成果
The research demonstrates that natural and bioinspired magnetic nanoparticles are effective multimodal contrast agents for photoacoustic and photothermal applications, enabling high-contrast detection and therapy of cancer cells with low laser energy. Ultrasharp resonances in vivo allow for super-resolution flow cytometry, highlighting their potential for clinical use in theranostics.
研究不足
The study is limited by the complexity of purifying nMNPs from bacterial debris, potential toxicity of GNRs at high concentrations, and the need for further optimization for clinical translation. In vivo studies were conducted in mouse models, which may not fully replicate human physiology.
1：Experimental Design and Method Selection:
The study employed photoacoustic (PA) and photothermal (PT) cytometry, spectroscopy, and flow cytometry integrated with magnetic manipulation, dark-field microscopy, and transmission electron microscopy. Methods were selected to assess PA and PT properties, cellular uptake, and in vivo applications.
2：Sample Selection and Data Sources:
Magnetospirillum magneticum AMB-1 bacteria were cultured; nMNPs were isolated and purified. Human breast cancer cells (MDA-MB-231) and mouse leukocytes were used. In vivo studies involved nude mice.
3：List of Experimental Equipment and Materials:
Equipment included a multifunctional integrated PA and PT platform, ultrasound bath (Model 2200, Branson), magnetic MS Columns (MACS, Miltenyi Biotec), spectrophotometer (Ultrospec 3300 PRO, Amersham Biosciences), TEM (JEM 2100F, JEOL), and neodymium-iron-boron magnet (MAGCRAFT). Materials included GNRs from Nanopartz Inc., folate, 4-mercaptobenzoic acid, and various buffers.
4：Experimental Procedures and Operational Workflow:
MBs were cultured and nMNPs isolated; bioinspired hybrids (nMNP-GNRs) were synthesized. Cells were incubated with nanoparticles, and PA/PT measurements were conducted in vitro and in vivo. Magnetic manipulation was applied to enhance signals. Cell viability was assessed using Trypan Blue test.
5：Data Analysis Methods:
Data were analyzed using statistical methods (means, standard errors, t-tests with p≤0.05 significance), and software developed in-house for signal processing.
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