研究目的
To evaluate the thickness of choroid and retinal nerve fiber layer (RNFL) in Multiple Sclerosis (MS) patients with and without optic neuritis using enhanced depth imaging optical coherence tomography (EDI-OCT).
研究成果
RNFL thickness is reduced in MS patients compared to healthy controls and is thinner in MS-ON than MS-NON groups, with temporal RNFL thickness correlating negatively with EDSS score and disease duration, indicating its utility as a follow-up parameter. Choroidal thickness was thicker in MS-ON than MS-NON but not significantly different from controls, suggesting it is not a reliable indicator of axonal loss. Future studies should use larger groups and automated methods for EDI-OCT choroidal measurements.
研究不足
The cross-sectional design limits causal inferences. Examinations were not performed during the acute phase of optic neuritis. The choroidal thickness increase in MS-ON group compared to controls was insignificant and controversial, possibly due to limited sample size. Choroidal thickness measurements were manual due to lack of automated software algorithms, reducing standardization and reproducibility.
1：Experimental Design and Method Selection:
A cross-sectional study design was used. Enhanced depth imaging optical coherence tomography (EDI-OCT) was employed for choroidal and RNFL thickness measurements. Statistical analyses included chi-square test, one-way ANOVA, Tukey test, Mann-Whitney-U test, Pearson and Spearman correlation coefficients, and generalized estimating equation (GEE) models to account for within-subject correlation.
2：Sample Selection and Data Sources:
52 adult relapsing remitting MS patients (104 eyes) and 36 healthy control subjects (36 eyes) were selected. MS patients were grouped into those with optic neuritis (MS-ON) and without optic neuritis (MS-NON). Exclusion criteria included diabetes mellitus, systemic hypertension, cardiovascular disease, connective tissue disease, other neurological diseases, smoking history, less than 6 months after acute optic neuritis, ophthalmic surgery, refractive errors >3 diopters, and ocular pathologies affecting OCT measurements.
3：List of Experimental Equipment and Materials:
Spectralis? spectral-domain OCT (Heidelberg Engineering, Heidelberg, Germany) with Enhanced Depth Imaging (EDI) mode, Snellen chart for visual acuity, slit lamp for examination, and SPSS 22 statistical software (IBM SPSS Statistics; IBM, Armonk, N.Y., USA) for data analysis.
4：Experimental Procedures and Operational Workflow:
All participants underwent comprehensive ophthalmologic examinations including best corrected visual acuity, intraocular pressure measurement, anterior segment and fundus examination via slit lamp, and EDI-OCT scanning. Choroidal thickness was measured manually at subfoveal, 1500 μm nasal (N1500), and 1500 μm temporal (T1500) points from the fovea using 30° line scanning. RNFL thickness was measured automatically using a 3.6 mm circular scan around the optic nerve, with segmentation by Spectralis software.
5：6 mm circular scan around the optic nerve, with segmentation by Spectralis software.
Data Analysis Methods:
5. Data Analysis Methods: Data normality was confirmed with Kolmogorov-Smirnov test. Categorical variables compared with chi-square test; continuous variables with one-way ANOVA and post-hoc Tukey test. EDSS score and disease duration compared with Mann-Whitney-U test. Correlations assessed with Pearson or Spearman coefficients. GEE models used for ocular variables with age as covariate. Statistical significance set at p<0.05.
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