1：Experimental Design and Method Selection:

The study utilized atom transfer radical polymerization (ATRP) to synthesize a glucose-containing diblock copolymer (PEG-PGAMA) for drug delivery. The method was chosen for its ability to produce well-defined block copolymers. Dynamic chemical complexation based on pH-induced boronic acid-diol interactions was employed for drug loading and release.

2：Sample Selection and Data Sources:

The copolymer was synthesized from PEG113-OH and GAMA monomer. Bortezomib (BTZ) was used as the model drug. Samples were prepared in aqueous solutions and characterized using NMR, GPC, UV-Vis spectroscopy, and fluorescence spectrometry.

3：List of Experimental Equipment and Materials:

Materials included PEG113-OH, 2-bromoisobutyryl bromide, 2-aminoethyl methacrylic acid hydrochloride, D-glucono-lactone, BTZ, solvents like methanol, toluene, DMSO, and reagents such as triethylamine and CuBr. Equipment included a Bruker 400 MHz NMR spectrometer, GPC system with Waters components, PerkinElmer Lambda Fluorescence Spectrometer, and UV-Vis spectrophotometer.

4：Experimental Procedures and Operational Workflow:

Synthesis involved preparing GAMA monomer, PEG-Br macroinitiator, and PEG-PGAMA copolymer via ATRP. Drug loading was achieved by dissolving BTZ and copolymer in DMSO, adding NaOH, and dialyzing. Characterization steps included NMR for structure analysis, GPC for molecular weight, fluorescence spectrometry for complexation studies, and UV-Vis for drug quantification and release kinetics.

5：Data Analysis Methods:

Data were analyzed using standard curves from UV-Vis absorbance measurements to calculate drug loading capacity and release profiles. Fluorescence intensity changes were used to confirm complex formation. Statistical analysis relied on calibration with standards and reproducibility checks.