研究目的
To observe the microvascularization of the spinal cord and identify viable tissue during the repair of myelomeningocele using vascular fluorescence techniques.
研究成果
Vascular fluorescence with indocyanine green and fluorescein effectively allows observation of spinal cord microvascularization, aiding in the identification of viable neural tissue and minimizing injury during myelomeningocele repair. This technique enhances surgical precision and tissue preservation.
研究不足
The study is based on a single case, which may limit generalizability. Potential areas for optimization include larger sample sizes and quantitative analysis of fluorescence data.
1：Experimental Design and Method Selection:
The study uses microsurgical imaging with fluorescence techniques (fluorescein and indocyanine green) to visualize spinal cord microvascularization in a newborn with giant myelomeningocele.
2：Sample Selection and Data Sources:
A single case of a newborn with giant myelomeningocle is used, with images captured during surgery.
3：List of Experimental Equipment and Materials:
Fluorescein (2mg/Kg), indocyanine green (
4：5mg/Kg), and a fluorescence imaging system capable of detecting spectra at 550 nm and 800 nm. Experimental Procedures and Operational Workflow:
Intravenous administration of fluorescein and indocyanine green, followed by capture of fluorescence images at specified wavelengths to observe microvascular patterns and differentiate tissue types.
5：Data Analysis Methods:
Visual analysis of images to identify microvascular anatomy and differentiate between viable neural tissue and epithelial zones based on vascularization patterns.
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