1：Experimental Design and Method Selection:

The study designed various liposome formulations with different lipid compositions (anionic, cationic, pegylated) to incorporate GdDTPA1 and ZnPc. Methods included thin-film hydration for liposome preparation, nanoparticle tracking analysis for size, differential scanning calorimetry for thermal behavior, singlet oxygen quantum yield measurement, NMR relaxometry, cytotoxicity assays (MTT), and confocal microscopy for cellular localization.

2：Sample Selection and Data Sources:

Liposomes were prepared using specific lipids (POPC, PG, DOTAP, PEG-DPPE, GdDTPA1) and ZnPc. Cell lines used were HeLa (cervical cancer) and MSU-1.1 (normal fibroblasts).

3：1 (normal fibroblasts). List of Experimental Equipment and Materials:

3. List of Experimental Equipment and Materials: Equipment included NanoSight NS500 for NTA, DSC8000 calorimeter, JEOL JEM-1400 TEM, NMR spectrometers at 9.4 T and 0.39 T, high-pressure xenon lamp for irradiation, Biochrom Anthos Zenyth 340 spectrophotometer for MTT assay, Olympus FV confocal microscope. Materials were lipids from Avanti Polar Lipids, ZnPc from Sigma Aldrich, and cell culture reagents.

4：4 T and 39 T, high-pressure xenon lamp for irradiation, Biochrom Anthos Zenyth 340 spectrophotometer for MTT assay, Olympus FV confocal microscope. Materials were lipids from Avanti Polar Lipids, ZnPc from Sigma Aldrich, and cell culture reagents. Experimental Procedures and Operational Workflow:

4. Experimental Procedures and Operational Workflow: Liposomes were prepared by thin-film hydration and extrusion. Size was measured over time with NTA. Thermal behavior was assessed by DSC. Singlet oxygen production was measured using DPBF quenching. Relaxivity was determined via NMR. Cytotoxicity was tested with MTT assay after 24h incubation and light exposure. Cellular localization was imaged with confocal microscopy after staining.

5：Data Analysis Methods:

Data were analyzed using standard fitting for NMR relaxation times, statistical analysis for cytotoxicity (triplicates with standard deviation), and qualitative assessment for microscopy images.