1：Experimental Design and Method Selection:

The study involved preparing inclusion complexes of curcumin and THC with hydroxypropyl-β-cyclodextrin (HP-βCD) and hydroxypropyl-γ-cyclodextrin (HP-γCD) using a spray drying technique. Physicochemical characterization included FT-IR, DSC, XRD, DLS, TEM, and drug release studies. Cellular permeability was assessed using human corneal epithelial (HCE) and retinal pigmented epithelial (RPE) cells in Transwell inserts. Anti-oxidant efficacy was evaluated via qRT-PCR for SOD1, CAT1, and HMOX1 genes, and oxidative protection was tested in rabbit corneas using H2O2-induced stress.

2：Sample Selection and Data Sources:

Curcumin and THC were used as drug compounds. HCE cells were obtained from CellnTec, and RPE cells from ATCC. Rabbit corneas were excised from New Zealand white rabbits.

3：List of Experimental Equipment and Materials:

HPLC system (Waters Alliance), FT-IR spectrometer (JASCO FT-IR 6300), DSC (TA Instruments Q20), XRD (Rigaku Ultima IV), DLS (NanoBrook 90Plus), TEM (JEOL JEM 2100F), Transwell inserts (Corning), cell culture media (Invitrogen, CellnTech), qRT-PCR kits (Qiagen, QuantiTect, Enzynomics), and histological staining materials.

4：Experimental Procedures and Operational Workflow:

Drug complexes were prepared by dissolving HP-CDs in water, adding drug solutions in acetone, stirring overnight, centrifuging, and freeze-drying. Characterization involved FT-IR, DSC, XRD, DLS, TEM, and drug release studies. Cellular transport assays used HCE and RPE cells on Transwell inserts with TEER measurements. Cytotoxicity was assessed via WST-1 assay. Anti-oxidant gene expression was measured by qRT-PCR after H2O2 treatment. Rabbit corneas were treated with drug formulations and H2O2 in a diffusion apparatus, followed by H&E staining.

5：Data Analysis Methods:

Data were analyzed using GraphPad Prism 5.03 with unpaired Student's t-test and one-way ANOVA (Tukey's post hoc test). Gene expression data were analyzed using the 2-ΔΔCt method.