Study of interaction between citrate-coated silver nanoparticles and gamma globulin using spectroscopic method

摘要： Studies on the biomedical applications of silver nanoparticles (AgNPs) have attracted great attention during the past decades. In this paper, the citrate-coated AgNPs with uniform size and good monodispersity are synthesized. The diameter of the synthesized AgNPs is about 25.4 nm. Its interaction with γ-globulin was thoroughly studied using UV-vis absorption spectroscopy, fluorescence spectroscopy, fluorescence lifetime measurement, synchronous fluorescence spectroscopy, 3D fluorescence spectroscopy as well as CD spectroscopy. From the above experiments, we can obtain that citrate-coated AgNPs can bind to γ-globulin with high affinity. The fluorescence quenching of γ-globulin by AgNPs is in a static mechanism. Van der Waals interactions and hydrogen bonds play great role in the binding interaction. The interaction can induce slight conformational change of γ-globulin.