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[IEEE 2017 21st National Biomedical Engineering Meeting (BIYOMUT) - Istanbul (2017.11.24-2017.12.26)] 2017 21st National Biomedical Engineering Meeting (BIYOMUT) - Three Dimensional Bioprinting of Tissue Engineered Artificial Heart Valves by Stereolithography
摘要： Over the past several decades, there has been an ever-increasing demand for organ transplants. There are no significant increase observed in organ donations while the need for organ transplantation is keep increasing. Organ donation emerges as the most feasible solution especially in heart valve diseases. There are two types of artificial heart valves are utilized for heart valve replacement operations. These are mechanical heart valves and bioprosthetic heart valves. However, there are severe intrinsic problems exist for both type of artificial heart valves that are hard to be solved. Due to these reasons, growing or fabricating tissue engineered heart valves using biomaterial scaffolds with a person’s own cells with high biocompatability. The purpose of this study is; fabricating 3D artificial heart valves by stereolithography that has potential to be obtained for heart valve replacements. Stereolithography has proven potential for fabricating 3D models with high structural integrity and strong mechanical properties, yet it has not been evaluated as a bioprinter. In this study, this purpose is aimed to be succeeded.
关键词： 3D bioprinting,artificial heart valves,stereolithography,tissue engineering
更新于2025-09-23 15:21:21
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Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study
摘要： Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. The onset of both pancreatitis and pancreatic cancer follows a common pattern: exocrine pancreatic acinar cells undergo a transdifferentiation to duct cells that triggers a 3D restructuration of the pancreatic tissue. However, the exact mechanism underlying this process remains partially undefined. Further understanding the cellular events leading to PDAC could open new avenues in the development of novel therapeutic approaches. Since current 2D cell culture models fail to mimic the tridimensional complexity of the pancreatic tissue, new in vitro models are urgently needed. Here, we generated 3D pancreatic cell spheroid arrays using laser-assisted bioprinting (LAB) and characterized their phenotypic evolution over time through image analysis and phenotypic characterization. We show that these bioprinted spheroids, composed of both acinar and ductal cells, can replicate the initial stages of PDAC development. This bioprinted miniaturized spheroid-based array model should prove useful for the study of the internal and external factors that contribute to the formation of precursor PDAC lesions and to cancer progression, and may therefore shed light on future PDAC therapy strategies.
关键词： pancreatic cancer,Laser-assisted bioprinting,early lesions,in vitro cancer model,cancer initiation
更新于2025-09-19 17:13:59
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Rapid Prototyping of Biomaterials || Rapid prototyping of complex tissues with laser-assisted bioprinting (LAB)
摘要： In parallel with scaffold-based approaches involving cell seeding of porous structures, some authors have suggested that three-dimensional (3-D) biological structures can be built from the bottom up using the technology of bioprinting; the automated, computer-aided deposition of cells; cell aggregates; and biomaterials. To this end, inkjet printers have been successfully used to pattern biological assemblies according to a computer-aided design (CAD) template. Pressure-operated mechanical extruders have been also developed to handle living cells and cell aggregates. In parallel with these methods, laser-assisted printing technologies have emerged as alternative methods for the assembly and micropatterning of biomaterials and cells. Laser printing of biological material in general and living cells in particular is based on the laser-induced forward transfer (LIFT) technique in which a pulsed laser is used to induce the transfer of material from a source film to a receiving substrate. Several variations of LIFT have been implemented to print living cells. For convenience, we will use the general term of LAB, although we will mainly discuss results obtained with a setup that requires an intermediate light-absorbing layer of metal. Thus, under suitable irradiation conditions and for liquids presenting a wide range of rheologies, the material can be deposited in the form of well-defined circular droplets with a high degree of spatial resolution.
关键词： bioprinting,laser-assisted bioprinting,cell printing,tissue engineering,LIFT
更新于2025-09-12 10:27:22
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From PV to bio: Surviving the photovoltaic bubble at laser centre UPM
摘要： Laser Centre UPM is one of the leading Research Centers in Spain in the field of industrial applications of high power lasers. Founded in 1998 in the oldest and largest Spanish Technical University, Universidad Politécnica de Madrid, its activity is focused in technological transfer of laser-based processes to industry. With an important experience in laser micromachining using fast and ultrafast lasers, one of the fields in which its activity has produced more relevant results is laser process development for photovoltaic (PV) industry. Starting in 2004, its activity in this field in collaboration with other public Spanish institutions and other industrial partners has had a remarkable scientific and technological impact. Unfortunately, this activity was seriously restricted since 2010 due to the economic crisis in general and the photovoltaic industry evolution in particular. However, the background acquired in some particular applications and the inertia obtained with that activity has opened new fields of activity of Laser Center UPM in advanced laser-based processes for biomedicine. This paper summarizes the main achievements of Laser Center UPM in PV and how it performed the transition to new applications in a completely different sector, like biomedicine, using the experienced accumulated in the development of new laser based processes for PV.
关键词： metallization,laser bioprinting,LIFT,laser microprocessing,silicon photovoltaics
更新于2025-09-12 10:27:22
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[IEEE 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - Honolulu, HI (2018.7.18-2018.7.21)] 2018 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) - 3D Bioprinting of Cyanobacteria for Solar-driven Bioelectricity Generation in Resource-limited Environments
摘要： We demonstrate a hybrid biological photovoltaic device by forming a 3D cooperative biofilm of cyanobacteria and heterotrophic bacteria. 3D bioprinting technique was applied to engineer a cyanobacterial encapsulation in hydrogels over the heterotrophic bacteria. The device continuously generated bioelectricity from the heterotrophic bacterial respiration with the organic biomass supplied by the cyanobacterial photosynthesis. This innovative device platform can be the most suitable power source for unattended sensors, especially for those deployed in remote and resource-limited field locations.
关键词： 3D bioprinting,heterotrophic bacteria,unattended sensors,hybrid biological photovoltaic device,3D cooperative biofilm,cyanobacteria,bioelectricity
更新于2025-09-11 14:15:04