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Ultrasmall Au-Ag Alloy Nanoparticles: Protein-directed Synthesis, Biocompatibility and X-ray Computed Tomography Imaging ?
摘要： The ultrasmall sizes of nanoparticles have attracted significant attention for potential application in the fields of catalysis and nanomedicine. Herein, we reported on the green preparation and X-ray computed tomography (CT) imaging of ultrasmall bimetallic bovine serum albumin-directed gold-silver (Au-Ag@BSA) nanoparticles (2–4 nm) using BSA as a stabilizing and template-directed agent. Further, the effects of synthesis condition were systematically explored to prepare products by adjusting the different molar ratios of Au/Ag. The resulting Au-Ag@BSA nanoparticles exhibited the spherical shape, well-dispersed ability, as well as long-term room-temperature stability. The cytotoxicity effects of Au-Ag@BSA nanoparticles on A549 and MCF-7 cells were compared with those of individual Ag nanoparticles, and the results indicated lower cytotoxicity effect by Au-Ag@BSA nanoparticles. Furthermore, the in vivo toxicity of Au-Ag@BSA nanoparticles was investigated in the early-stage zebrafish embryos. The results indicate that there are not any obvious changes of survival and hatching percentages at multiple growth stages (4-120 hpf) even a high level of Au-Ag@BSA nanoparticles (up to 80 mM), revealing the good biocompatibility. Interestingly, a rational design of Au/Ag molar ratio (3:2) surprisingly possessed the enhanced CT performances compared to the Au nanoparticles and iohexol. Accordingly, this study highlights a new prospect in the green preparation of ultrasmall alloy nanomaterials with good biocompatibility and will be of great interest in developing CT contrast agent, catalyst as well as drug delivery carrier.
关键词： green synthesis,Au-Ag@BSA nanoparticles,biocompatibility,zebrafish embryos,enhanced CT imaging
更新于2025-09-23 15:23:52
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Super Blinking and Biocompatible Nanoprobes Based on Dye Doped BSA Nanoparticles for Super Resolution Imaging
摘要： As one of the super-resolved optical imaging technique, single molecule localization microscopy (SMLM) imaging techniques, SMLM has one particular request for the fluorophores, that is, continuous “on” and “off” behaviors of their signals (referred to as “blinking”). Hence, we present here a kind of Super Blinking and biocompatible Nanoprobes (denoted as SBNs) for SMLM. The SBNs have two main advantages, first, they possess an outstanding fluorescence blinking. Second, they are biocompatible since they are based on bovine serum albumin (BSA). The SBNs are fabricated by doping organic dyes into BSA nanoparticles (NPs). We fabricated two kinds of SBNs, one was doped with Alexa Fluor 647 (A647) and the other was doped with Alexa Fluor 594 (A594). Especially for A594 doped SBNs, the improved blinking of A594 doped SBNs induced a better localization precision as compared with A594 alone. Moreover, SMLM imaging of breast cancer cells and exosomes using the SBNs was successfully realized with high spatial resolutions. The work demonstrated here provides a new strategy to prepare novel kinds of super blinking fluorescent agents for SMLM, which broadens the selection of suitable fluorophores for SMLM.
关键词： BSA nanoparticles,single molecule localization microscopy,super blinking,dyes,localization precision
更新于2025-09-10 09:29:36