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Anti-Reflecting Absorbing Layers for Electrochemical and Biophotonic Applications
摘要： Anti-reflecting layers deposited on flat surfaces make molecular films visible in reflecting light microscopy. For centuries, single Anti-Reflecting layers have been implicitly associated with dielectric materials. We recently demonstrated that anti-reflecting layers could be achieved out of absorbing materials such as metals as well, but only when used as backside layers where illumination and detection are performed through a supporting window. Fortunately, this corresponds to the best geometry when envisaging biophotonic or electrochemical applications at the solid/liquid interface. Here we explain how single absorbing anti-reflecting layers can serve each of these applications, and both simultaneously.
关键词： Biophotonic,Zeolites,Anti-Reflecting,Electrochemical,applications
更新于2025-09-23 15:23:52
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Photodynamic Therapy at Low-Light Fluence Rate: in vitro Assays on Colon Cancer Cells
摘要： This paper presents the results of in vitro photodynamic therapy assays on RKO and HCT-15 cell lines. The envisaged implementation is in autonomous medical microdevices, such as endoscopic capsules for clinical treatment of several types of gastrointestinal tract tumors. Because of their very limited device volume, light fluence and fluence rate needed to destroy tumor cells should be minimized. Foscan or meta-tetra(hydroxyphenyl)chlorin (mTHPC) is used as a photosensitizer. The experimental results show that a small amount of mTHPC (0.15 mg/kg) and light fluence (5–20 J/cm2) is sufficient to obtain significant photodynamic activity. An array of LEDs with peak transmittance at 652 nm is used as a portable light source for the maximum quantum efficiency in producing singlet oxygen. Irradiation to a light fluence between 2.5 and 10 J/cm2 is achieved by an increased exposure time at an 11 mW/cm2 light fluence rate, while mTHPC concentrations of 0.5, 1, 5, and 10 μg/mL are used. The experimental results show that decreased cell viability (down to 30%) can be obtained for 1–5 μg/mL of mTHPC concentrations and 2.5 J/cm2 of light fluence. Such light fluence and light fluence rate are compatible with the endoscopic capsules batteries.
关键词： Biophotonic therapeutic technique,Photodynamic therapy,light-tissue interaction
更新于2025-09-23 15:22:29
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Silicon Photomultipliers and SPAD imagers in biophotonics: Advances and perspectives
摘要： Photonics is essential in life science research and the continuous development of methods offers researchers tools of unprecedented sensitivity. Sensors are key to the exploitation of the most advanced biophotonic techniques with highly demanding specifications in terms of single photon sensitivity, time resolution, miniaturization real-time processing and data throughput. Silicon photomultipliers and Single Photon Avalanche Diode (SPAD) imagers represent the state-of-the-art in photon detection with single photon sensitivity, photon number resolving capability and the possibility to integrate on chip advanced functionalities. As a consequence, they can be the platform for the next generation biophotonic instruments and methods. This paper summarises the main biophotonic techniques and reports exemplary applications of Silicon Photomultipliers and SPAD imagers for fluorescence, chemiluminescence, time correlated single photon counting and imaging. Achievements and current limitations are addressed, pointing as well to the most recent technology advances and highlighting the possible pathways for the near future.
关键词： SPAD imagers,Biophotonic techniques,Silicon Photomultipliers
更新于2025-09-23 15:21:21
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Fabrication and Characterization of New Phosphate Glasses and Glass-Ceramics Suitable for Drawing Optical and Biophotonic Libers
摘要： Rare earth doped phosphate glasses are attractive materials for the engineering of photonic devices, due to their easy processing, good thermal stability, excellent optical properties and high rare-earth ions solubility [1]. Besides, phosphate glasses with a P2O5 content of 50 mol% have been shown to be suitable for fiber drawing. It is well known that if the rare-earth ions are located in crystalline phase of desired nature and structure, the spectroscopic properties of the glasses can be enhanced [2]. Therefore, efforts have been focused on the development of new glass-ceramics (GCS) obtained from the heat treatment of glasses, as these engineered materials possess some of the glass properties (large flexibility of composition and geometry) but also some advantages of the RE-doped single crystals (high absorption and emission as well as long lifetimes). In this presentation, we will first discuss how new active phosphate glasses can be prepared with a bioactivity functionality and their composition tailored to enhance their spectroscopic properties. We will show that the heat treatment does not necessarily lead to the bulk precipitation of rare-earth doped crystals (see figure 1). Therefore, we will present a new route to prepare rare-earth doped crystals containing glasses using the direct doping method (schematic presented in Figure 2). We will review the main challenge with this novel route of preparing glasses, related to the survival and dispersion of the particles in the glasses. Finally, we will demonstrate that some of the promising glasses can be drawn into optical fibers with broad luminescence over 70 nm of bandwidth and also into biophotonic fiber in the prospect of developing an innovative biosensor.
关键词： glass-ceramics,biophotonic fibers,phosphate glasses,rare-earth ions,optical fibers
更新于2025-09-23 15:19:57
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Biophotonic pretreatment enhances the targeting of senile lentigines with a 694 nm QS‐ruby laser
摘要： The ability of lasers to selectively destroy the melanin pigment responsible for solar/senile lentigines (SL) (age spots) without damaging the surrounding tissue makes laser therapy a preferred option for treating SL. However, laser therapies typically utilize a high energy output to achieve thermal ablation of their selective targets – and therefore can be associated with a period of recovery, healing and down time, depending on the modality. The Kleresca? biophotonic platform which generates fluorescent light energy (FLE), is a non-invasive form of photobiomodulation with multiple applications. FLE has clinical efficacy in treating inflammatory skin conditions such as acne vulgaris and rosacea, in addition to its healing and normalizing properties, rejuvenating the skin. It has previously been combined with other invasive treatment techniques; for example, following picosecond laser treatment of SL, where it enhanced the overall appearance of the skin. A common observation with the Kleresca? biophotonic platform is the transient emergence of underlying hyperpigmented spots. This case sought to investigate the use of the Kleresca? treatment to intensify and demask underlying SL prior to their targeting with laser therapy.
关键词： senile lentigines,biophotonic pre-treatment,694 nm QS-ruby laser,Kleresca? biophotonic platform,photobiomodulation
更新于2025-09-11 14:15:04