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Peptide-based ratiometric fluorescent probe for highly selective detection of Cd (II) and its application in bioimaging
摘要： This work reported a new peptide fluorescent probe L including dansyl group (acceptor) and tryptophan (donor) via imitated the binding sites of natural protein. L exhibited exclusively selective ratiometric fluorescence response towards Cd2+ ions over other metal ions based on fluorescent resonance energy transfer (FRET) effects. As designed, L showed exciting sensitivity to Cd2+ ions in aqueous media, and the detection limits are less than 27.5 nM. In addition, the binding pattern of L and Cd2+ was confirmed by fluorescence titration analysis which was found to be 2:1 and further it was confirmed by Job's plot. What' more, L exhibited very low biotoxicity and exciting cell infiltration, and have been successfully used for fluorescence imaging of Cd2+ ions in living HeLa cells.
关键词： Cell imaging,Fluorescent probe,Peptide,Cadmium ion,Ratiometric
更新于2025-09-23 15:23:52
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Colorimetric and dark-field microscopic determination of cadmium(II) using unmodified gold nanoparticles and?based on the formation of glutathione-cadmium(II) complexes
摘要： A colorimetric approach is presented for the determinaton of cadmium(II) using unmodified gold nanoparticles (AuNPs) as a colorimetric probe. The assay is based on AuNP aggregation that is induced by the complexes formed between Cd(II) and glutathione (GSH) in a concentrated solution of NaCl. Unmodified AuNPs are known to aggregate in high-salt medium, but GSH can prevent aggregation. In the presence of Cd(II), it will bind GSH, and this will cause the AuNPs to aggregate as indicated by yellow and red dots under dark-field microscopy observation and the formation of a blue coloration. By monitoring the intensity change of AuNPs (as a ratio of absorbances at 600 and 520 nm), Cd(II) can be quantified with a linear response in the 17 pM to 16.7 nM concentration range and a detection limit of 4.3 pM. The method was successfully applied to the determination of Cd(II) in spiked lake water by the standard addition mode, and the detection limit is 4.5 pM.
关键词： Microscopy,Yellow and red dots,Lake water,Glutathione,Ratiometric detection,Cadmium ion,Gold nanoparticle aggregation
更新于2025-09-23 15:19:57