- 标题
- 摘要
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- 实验方案
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Correlated Secondary Ion Mass Spectrometry-Laser Scanning Confocal Microscopy Imaging for Single Cell-Principles and Applications
摘要： Secondary ion mass spectrometry (SIMS) as a powerful surface analysis technique has been widely applied in semiconductor industry and geology research. Recently, with the development of instrumental technology, SIMS is attracting more and more attention in life sciences. SIMS can provide surface MS spectra, 2D/3D chemical images and depth profiling of substances simultaneously. The minimal lateral resolution of 2D SIMS imaging is 80?100 nm, and the longitudinal resolution of 3D SIMS imaging is about 1–5 nm. However, owing to lack of specific ions to render the structures of organelles, SIMS imaging for single cells still have great challenges. Optical microscopy, in particular laser scanning confocal microscopy (LSCM), has been emerged to be an indispensable technique for single cell imaging and can obtain high spatial 2D/3D imaging to visualize the structures of organelles. Thus, the combinational use of SIMS and LSCM, which takes advantages of SIMS for molecular imaging and LSCM for morphological imaging, has greatly extended the application of SIMS imaging and ensured its accuracy at single cells level, providing novel insights into better understanding of the biological events inside cells. In this review, we focus on the development and application of SIMS imaging and the correlated SIMS and LSCM imaging in the research of cell biology and drug discovery. We anticipate that the combinational use of SIMS and LSCM imaging has promising future in biomedicine and life sciences.
关键词： Cell biology,Single cell imaging,Laser scanning confocal microscopy,Correlated secondary ion mass spectrometry and laser scanning confocal microscopy imaging,Secondary ion mass spectrometry,Review
更新于2025-09-16 10:30:52
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Application of PEGylated graphene quantum dots in cell imaging
摘要： Polyethylene glycol (PEG) functionalized graphene quantum dots (GQDs-PEG) with average particle size of 2.1 nm were successfully prepared by hydrothermal method with PEG as surface passivation agent. The quantum yield (QY) of GQDs-PEG was 3.7%and its physiological stability was significantly better than that of GQDs. The cell viability assay results showed that GQDs-PEG display no obvious toxicity to cells. The results of cell fluorescence imaging showed that GQDs-PEG could enter the whole cell with high fluorescence recognition.
关键词： cell imaging,graphene quantum dots,Polyethylene glycol
更新于2025-09-11 14:15:04
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Carbon dots as a dual sensor for the selective determination of d-penicillamine and biological applications
摘要： Dual mode Nanosensors gained tremendous interest in recent years because of their facile synthesis and dual applications. Herein we have synthesized highly fluorescent carbon dots (CDs) from Mahogany fruit shell by chemical oxidation method. The as prepared CDs exhibited selective and sensitive quenching of fluorescence by Fe3+ and also accompanied by a dramatic increase in absorption intensity. Hence these two processes led to fabricate CDs-Fe3+ system as a dual probe. D-Penicillamine (D-PA) has much affinity toward Fe3+ which resulted in recovery of almost 75% fluorescence intensity and decrease in absorption of the CDs-Fe3+ system. Thus, this tendency has been exploited for the selective detection of D-PA by both Spectrofluorimetrically and UV-Visible spectroscopically and showed a wide linear range of detection 0-48 μg mL?1 respectively. This developed probe offered low cost, high selectivity, repeatability, facile operation and excellent recovery ratio in detection of D-PA in pharmaceutical samples. Moreover, biological applications CDs were investigated using Saccharomyces cerevisiae strain with confocal microscopy. We found that CDs were a biocompatible and ideal candidate for differential staining of yeast cells.
关键词： D-penicillamine detection,Cell imaging,Carbon dots (CDs),Dual sensor,Biocompatibility,UV-Visible and spectrofluorimetrically
更新于2025-09-10 09:29:36
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Spray-Assisted Coil-Globule Transition for Scalable Preparation of Water-Resistant CsPbBr <sub/>3</sub> @PMMA Perovskite Nanospheres with Application in Live Cell Imaging
摘要： Despite their impressive optical properties, lead halide perovskite quantum dots (PQDs) have not realized their potential, especially in bioimaging applications, as they suffer from poor moisture and thermal stability, solvent incompatibility, and significant toxicity. Here, a spray-assisted coil–globule transition method for encapsulating CsPbBr3 (CPB) PQDs into poly(methyl methacrylate) (PMMA) polymer nanospheres is reported. Polyvinylpyrrolidone-capped CPB PQDs are synthesized via the ligand assisted reprecipitation method in dichloromethane. After dissolving PMMA, the above precursor solution is sprayed into petroleum ether under high pressure N2. High-pressure nebulization restricts the interactions between PMMA polymer chains, resulting in the formation of ≈112 nm nanoscale composite spheres after a coil–globule transition. The CPB@PMMA nanospheres not only possess 73% quantum yields but retain 81% of fluorescence intensity after the exposure to water for over 80 days. Due to their confined size and biocompatible encapsulation, they are readily available for cellular uptake and exhibit no toxicity on live HeLa cells. Furthermore, the PMMA surface allows for functional surface modification, carrying the possibility of targeting specific biological species and processes.
关键词： cell imaging,spray,quantum dots,coil–globule transition,perovskite
更新于2025-09-10 09:29:36
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Confined electrochemiluminescence in vertically ordered silica mesochannels for the imaging of hydrogen peroxide released from single cells
摘要： Here, the confined electrochemiluminescence (ECL) of luminol and hydrogen peroxide is achieved in vertically ordered silica mesochannels (SMCs) and applied to map the efflux of hydrogen peroxide from single living cells. The vertical alignment of SMCs on indium tin oxide (ITO) slides restricts the lateral diffusion of species generated in the ECL process. Accordingly, the cross-talk of luminescence intensity from nearby microregions is minimized for higher-spatial-resolution ECL imaging. The characterization of luminol ECL on these SMC-coated slides yields a detection range of hydrogen peroxide between 5 μM and 1 mM, providing high sensitivity in imaging the efflux of hydrogen peroxide from cells. Compared with the luminescence from individual cells on bare ITO slides, ~ 4-fold intensity is observed for SMC-coated ITO, suggesting a better electrode surface for single-cell ECL imaging.
关键词： single-cell imaging,hydrogen peroxide,silica mesochannels,confined electrochemiluminescence,luminol
更新于2025-09-10 09:29:36
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A fluorescent and colorimetric Schiff base chemosensor for the detection of Zn2+ and Cu2+: Application in live cell imaging and colorimetric test kit
摘要： A novel Schiff base chemosensor HMID, ((E)-1-((2-hydroxy-3-methoxybenzylidene)amino)imidazolidine-2,4-dione), have been designed and synthesized. Sensor HMID showed a selectivity to Zn2+ through fluorescence enhancement in aqueous solution. Its detection limit was analyzed as 11.9 μM. Importantly, compound HMID could be applied to image Zn2+ in live cells. Detection mechanism of Zn2+ by HMID was suggested to be an effect of chelation-enhanced fluorescence (CHEF) by DFT calculations. Moreover, HMID could detect Cu2+ with a change of color from colorless to pink. The selective detection mechanism of Cu2+ by HMID was demonstrated to be the promotion of intramolecular charge transfer band by DFT calculations. Additionally, HMID could be employed as a naked-eye colorimetric kit for Cu2+. Therefore, HMID has the ability as a 'single sensor for dual targets'.
关键词： chemosensor,zinc ion,cell imaging,fluorescent,colorimetric,copper ion
更新于2025-09-10 09:29:36
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Phenanthrene-imidazole-based fluorescent sensor for selective detection of Ag+ and F? ions: real sample application and live cell imaging
摘要： 4-Methyl-2,6-bis(1H-phenanthro[9,10-d]imidazol-2-yl)phenol (probe-MPIP) conjugate has been designed as a selective fluorescent probe for silver (Ag+) ions. The probe-MPIP exhibits selectivity towards Ag+ in DMSO-H2O (1:9 v/v) in 50 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) at pH 7.4. Moreover, the detection of probe-MPIP towards Ag+ ions could be realized with a “naked eye” with a color conversion from greenish yellow to orange. The linear relationship between fluorescence intensity and Ag+ ion concentration indicates that probe-MPIP can be used for quantification of Ag+ ions. The probe-MPIP can be used in a broad range of pH from 3 to 11. The limit of detection (LOD) and association constant (Ka) of MPIP-Ag+ complex have been determined and found to be 1.8 nM and 8.24 × 105 M?1, respectively, according to fluorescence titrations. Moreover, the binding ratio of probe-MPIP and Ag+ was found to be 1:1 by Job’s method. Furthermore, MPIP-Ag+ complex subsequently acted as a secondary fluorescent turn-on probe for fluoride (F?) ion recognition among the other anions. To assess the real-world efficacy of probe-MPIP and MPIP-Ag+ complex, a real water sample of silver and fluoride ions (Ag+ and F?) was evaluated. Furthermore, probe-MPIP and MPIP-Ag+ complex have been successfully applied to cell imaging studies.
关键词： Fluorescent probe,Silver and fluoride ion detection,Binding constant,In vitro cell imaging,Real sample analysis
更新于2025-09-10 09:29:36
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Gold Conjugated Carbon Dots Nano Assembly: FRET Paired Fluorescence Probe for Cysteine Recognition
摘要： The detection and discrimination of Cys amino acid from numerous other related biomolecules has great importance in clinical field for diagnosis of various diseases. Herein, to detect the Cys, we embedded the carbon dots (CDs), gold, and naphthalimide (L1) into a single ratiometric fluorescence sensor assembly. Sensor assembly works on the principle of FRET mechanism between CDs and naphthalimide when CDs and L1 adhered on gold nanoparticles surface. Gold metal was turned into solid support by in situ reduction of HAuCl4 in the presence of CDs and L1. When the assembly was excited at 360 nm, emission maxima at 568 nm corresponded to naphthalimide emission was emerged that signifies the existence of a FRET between the CDs and naphthalimide fluorophores. With the addition of Cys, the FRET mechanism eliminated and the change in the fluorescence emission at two different wavelengths (450 nm and 568 nm) was recorded. The endogenous images of Cys was recorded by collecting the fluorescence images of HeLa cells under fluorescence microscope and also applied for the assay of Cys in blood serum. Cytotoxicity studies of CDs and sensor assembly were evaluated by performing the MTT assay.
关键词： Cell Imaging,Fluorescence,Carbon dots
更新于2025-09-10 09:29:36
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Construction of acetaldehyde-modified g-C3N4 ultrathin nanosheets via ethylene glycol-assisted liquid exfoliation for selective fluorescence sensing of Ag+
摘要： We successfully prepared acetaldehyde-modified g-C3N4 ultrathin nanosheets (ACNNSs) by a simple ethylene glycol-assisted liquid exfoliation method. The introduction of acetaldehyde regulated the surface energy of g-C3N4 to better match with that of water, which improved the exfoliation efficiency. Moreover, the acetaldehyde introduces defects into the g-C3N4 structure, which can act as excitation energy traps and cause considerable variation in the fluorescence emission. Benefiting from the stable photoluminescence (PL) emission, good water solubility and biocompatibility, the obtained ACNNSs showed a selective fluorescent response to Ag+ both in aqueous solution and living cells. The strong absorption and intimate contact with Ag+, and its appropriate redox potential of ACNNSs contributed to this excellent fluorescent response. A simple and environmental friendly approach was proposed to simultaneously achieve modification and exfoliation of g-C3N4 in aqueous solution. These findings might lead to wider applications of carbon-based nanomaterials as active materials for fluorescence detection in the environment.
关键词： Graphitic carbon nitride nanosheets,Living-cell imaging,Glycol-assisted exfoliation,Fluorescent sensor,Acetaldehyde-modified,Ag+
更新于2025-09-10 09:29:36
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Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy
摘要： Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes of live cells remains a challenging problem. In this paper, we propose a multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination microscopy with multi-angle evanescent light illumination. We demonstrate that our approach of multi-angle interference microscopy is perfectly adapted to studying subcellular dynamics of mitochondria and microtubule architectures during cell migration.
关键词： super-resolution microscopy,live-cell imaging,multi-angle interference microscopy,mitochondria,microtubules
更新于2025-09-10 09:29:36