- 标题
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[Methods in Molecular Biology] Microfluidic Electrophoresis Volume 1906 (Methods and Protocols) || Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications
摘要： This chapter describes the development of paper-based microchip electrophoresis (pME) devices for the separation of clinically relevant compounds. pME were fabricated by laser cut and thermal lamination process using polyester pouches. In addition, hand-drawn pencil electrodes were integrated to the device to perform capacitively coupled contactless conductivity detection (C4D). Finished device costs less than US$ 0.10 and did not require either sophisticated instrumentation or clean room facilities. Furthermore, pME is lightweight, easy to handle, ?exible, and robust. pME-C4D device revealed an excellent capacity to separate BSA and creatinine in less than 150 s with baseline resolution. The device proposed in this chapter has proven to be a good alternative as a platform for the diagnosis of diseases from renal disorders such as diabetes mellitus and heart disease.
关键词： Biomolecules,Paper electrophoresis,Kidney failure,Pencil electrodes,Bovine serum albumin,Creatinine,Clinical diagnosis,Contactless conductivity detection
更新于2025-09-23 15:21:01
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A sensitive photochemical reaction-capacitively coupled contactless conductivity detection system for HPLC and its application in determination of Cyclosporin A
摘要： High performance liquid chromatography (HPLC) post-column photochemical reaction (PR) coupled capacitively coupled contactless conductivity detector (C4D) was used for the first time in analysis of weak ultraviolet (UV)-absorbing, non-fluorescence and nonpolar compound. A series of conditions including the radiation power of light source, the length of the reaction tube and the thickness of detection tube were investigated. HPLC-PR-C4D system was successfully applied to the determination of Cyclosporin A (CsA). Consequently, under optimal conditions, the detection system exhibited a detection limit of 0.04 μg/mL and wide linear range from 0.5 μg/mL to 100 μg/mL for CsA detection. Application of the HPLC-PR- C4D system to pharmaceutical formulation and biological samples revealed the system developed maybe reliably applied to clinical studies.
关键词： Photochemical reaction,Cyclosporin A,HPLC,Capacitively coupled contactless conductivity detection,Application
更新于2025-09-11 14:15:04