- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Photo‐controlled Dopamine Polymerization on DNA Origami with low Nanometer Resolution
摘要： Temporal and spatial control over polydopamine formation on the nanoscale can be achieved by installing an irradiation-sensitive polymerization system on DNA origami. Precisely distributed G-quadruplex structures on the DNA template serve as anchors for embedding the photosensitizer protoporphyrin IX, which — upon the irradiation with visible light — induces a multistep oxidation from dopamine to polydopamine, producing polymeric structures on designated areas within the origami framework. The photochemical polymerization process allows the exclusive control over polydopamine layer formation, which is adjusted by simply switching the light source on and off. The obtained polymer–DNA hybrid material shows significantly enhanced stability properties paving the way for biomedical and chemical applications that are commonly prohibited by the sensitivity of DNA.
关键词： DNA origami,DNA nanotubes,Photopolymerization,Polydopamine,Stability in water
更新于2025-09-11 14:15:04
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Photo-controlled Dopamine Polymerization on DNA Origami with low Nanometer Resolution
摘要： Temporal and spatial control over polydopamine formation on the nanoscale can be achieved by installing an irradiation-sensitive polymerization system on DNA origami. Precisely distributed G-quadruplex structures on the DNA template serve as anchors for embedding the photosensitizer protoporphyrin IX, which — upon the irradiation with visible light — induces a multistep oxidation from dopamine to polydopamine, producing polymeric structures on designated areas within the origami framework. The photochemical polymerization process allows the exclusive control over polydopamine layer formation, which is adjusted by simply switching the light source on and off. The obtained polymer–DNA hybrid material shows significantly enhanced stability properties paving the way for biomedical and chemical applications that are commonly prohibited by the sensitivity of DNA.
关键词： DNA origami,DNA nanotubes,Photopolymerization,Polydopamine,Stability in water
更新于2025-09-11 14:15:04
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Compact quantum dot surface modification to enable emergent behaviors in quantum dot-DNA composites
摘要： Quantum dot (QD) biological imaging and sensing applications often require surface modification with single-stranded deoxyribonucleic acid (ssDNA) oligonucleotides. Furthermore, ssDNA conjugation can be leveraged for precision QD templating via higher-order DNA nanostructures to exploit emergent behaviors in photonic applications. Use of ssDNA-QDs across these platforms requires compact, controlled conjugation that engenders QD stability over a wide pH range and in solutions of high ionic strength. However, current ssDNA-QD conjugation approaches suffer from limitations, such as the requirement for thick coatings, low control over ssDNA labeling density, requirement of large amounts of ssDNA, or low colloidal or photostability, restraining implementation in many applications. Here, we combine thin, multidentate, phytochelatin-3 (PC3) QD passivation techniques with strain-promoted copper-free alkyne-azide click chemistry to yield functional ssDNA-QDs with high stability. This process was broadly applicable across QD sizes (i.e., λem = 540, 560, 600 nm), ssDNA lengths (i.e., 10–16 base pairs, bps), and sequences (poly thymine, mixed bps). The resulting compact ssDNA-QDs displayed a fluorescence quenching efficiency of up to 89% by hybridization with complementary ssDNA-AuNPs. Furthermore, ssDNA-QDs were successfully incorporated with higher-order DNA origami nanostructure templates. Thus, this approach, combining PC3 passivation with click chemistry, generates ssDNA-PC3-QDs that enable emergent QD properties in DNA-based devices and applications.
关键词： ssDNA,click chemistry,DNA origami,quantum dots,fluorescence quenching
更新于2025-09-11 14:15:04
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DNA-Based Super-Resolution Microscopy: DNA-PAINT
摘要： Super-resolution microscopies, such as single molecule localization microscopy (SMLM), allow the visualization of biomolecules at the nanoscale. The requirement to observe molecules multiple times during an acquisition has pushed the field to explore methods that allow the binding of a fluorophore to a target. This binding is then used to build an image via points accumulation for imaging nanoscale topography (PAINT), which relies on the stochastic binding of a fluorescent ligand instead of the stochastic photo-activation of a permanently bound fluorophore. Recently, systems that use DNA to achieve repeated, transient binding for PAINT imaging have become the cutting edge in SMLM. Here, we review the history of PAINT imaging, with a particular focus on the development of DNA-PAINT. We outline the different variations of DNA-PAINT and their applications for imaging of both DNA origamis and cellular proteins via SMLM. Finally, we reflect on the current challenges for DNA-PAINT imaging going forward.
关键词： DNA PAINT,SMLM,DNA origami,DNA,fluorescence microscopy,super-resolution microscopy
更新于2025-09-04 15:30:14