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Treatment of Non-Segmental Vitiligo with Narrowband UVB Phototherapy (311 Nm): Clinical Efficacy and Mechanisms of Action
摘要： Objective: To estimate efficacy of narrowband UVB phototherapy (311 nm) in non-segmental vitiligo and its effect on subpopulations of T-cells and dendritic cells in vitiligo lesions. Methods: 56 patients with progressive non-segmental vitiligo underwent narrowband UVB phototherapy (311 nm). 11 patients had biopsies before and after therapy from lesional (depigmentation area), marginal, and perilesional skin for immunohistochemical studies of CD4+, CD8+, CD1a+ and CD83+ cells in epidermis and derma. Results: Median number of UVB procedures per course was 71, median total radiation dose-91.8 J/cm2, median percent of repigmentation-49%. Repigmentation of >25% of the affected area was reached in 67.9% of patients and repigmentation of 76-100% in 19.6% . VIDA index decreased from 2 to 1 post-treatment (P<0.035). In a subgroup who underwent immunohistochemical studies median number of UVB procedures per course was 88, median total dose of radiation-140.2 J/cm2, and median percent of repigmentation-50%. Significant activation of T-cellular immune reactions was found in lesions before treatment. There was a statistically significant increase in CD8+ lymphocytes and CD1a+ dendritic cells in marginal and perilesional normally pigmented skin in epidermis. There was an increase in CD4+ and CD8+ lymphocytes and CD83+ dendritic cells in all three zones in derma. Normalization of CD8+ and CD1+ cells in epidermis of the affected skin was observed post-treatment. There was only partial reduction of CD4+, CD8+ and CD1+ cells in dermis. Conclusions: Narrowband UVB phototherapy (311 nm) is an effective method of treatment of nonsegmental vitiligo. T-lymphocytes and Langerhans cells play a critical immunoregulatory role in narrowband UVB phototherapy (311 nm)-induced immune suppression. Lack of full normalization of immunological parameters in the skin after treatment indicates a need for optimization of narrowband UVB phototherapy (311 nm): conduction of longer courses including 150-200 procedures, or combinations of this treatment with immunosuppressive drugs.
关键词： Dendritic cells,Narrowband UVB phototherapy (311 nm),T-lymphocytes,Vitiligo,Immunohistochemical analysis
更新于2025-09-23 15:21:01
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OMIP‐0XX – 28‐color flow cytometry panel to characterize B cells and myeloid cells
摘要： This 28-color ?ow cytometry panel focuses on B cells, dendritic cells, and monocytes and was optimized for cryopreserved peripheral blood mononuclear cells (PBMC). In addition to markers enabling the analysis of monocytes (CD14) and de?nition of subsets within B cells (CD10, CD19, CD20, CD21, CD27, IgD, IgM) and dendritic cells (CD1c, CD11c, CD123, CD141, HLA-DR), we included functional markers such as chemokine receptors (CXCR3, CXCR5), surface immunoglobulins (IgA, IgD, IgG, IgM), Fc receptors (CD16, CD23, CD32, CD64), inhibitory molecules (CD73, CD85j), the co-stimulatory molecule CD40, and cytokine receptors (IL-21R, BAFF-R, TACI), which enables in-depth characterization of B cells, dendritic cells, and monocytes.
关键词： 28-color panel,B cells,dendritic cells,monocytes,flow cytometry
更新于2025-09-23 15:19:57
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MicroRNA Quantitation During Dendritic Cell Endocytosis Using Imaging Flow Cytometry: Key Factors and Requirements
摘要： Background/Aims: MicroRNA (miRNA)-induced suppression of dendritic cells (DCs) has been implicated in many diseases. Therefore, accurate monitoring of miRNA endocytosis by DCs is important for understanding the role of miRNAs in many diseases. Recently, a method for measuring the co-localization of Argonaute 2 (AGO2)-associated miRNAs on laser-scanning confocal microscopy method was proposed to localize the miRNAs. But its definition was limited by the number of observed cells through its accuracy. Methods: In this study, a method based on imaging flow cytometry was developed to localize miR-590-5p with fluorescent probes in DCs. miR-590-5p proven to play an important role in tumor immunity. This method enabled the quantification, visualization and localization of the fluorescence intensity in 30,000 individual cells. Results: Using this method, the DCs with different endocytotic ability were distinguished. The behaviour of miR-590-5p during endocytosis under the stimulation of tumor antigen in DCs was observed, binding to its cognate target mRNA and degradation in DCs. Conclusion: This method based on imaging flow cytometry provide an additional method to study miRNA processing in DCs, which makes it a valuable addition to existing miRNA research techniques
关键词： miR-590-5p,miRNA endocytosis,Flow cytometry,Dendritic cells,Argonaute 2 (AGO2),FRET analysis
更新于2025-09-10 09:29:36
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Cytotoxicity and Intracellular Uptake of Fluorescent BSA-AuNCs from Human Monocyte-Derived Immature Dendritic Cells
摘要： Dendritic cells (DCs) are one of the most effective antigen presenting cells (APCs), activating na?ve T and B lymphocytes to initiate adaptive immune responses. The engineering of nano-spheres, emulsions or tubules, holds a key for the development of new immunomodulatory agents to either effectively modulate or deliver immunologically active components to target sites. Bovine Serum Albumin capped gold nanoclusters (BSA-AuNCs) are one of the most promising protein based nanoclusters because of their biological compatibility, ultrafine size, large stokes shift, and photoluminescence characteristics. AuNCs stabilized by BSA have recently been investigated for their use as drug carriers and tracking agents for bioimaging and biolabeling. BSA-AuNCs can be delivered to cells with target specificity for therapeutic applications both in vitro and in vivo because of their salient features including biodistribution, targeted binding, high clearance, less toxicity, and well suitability biologically. The present study demonstrated the less cytotoxic response of stable and highly fluorescent BSA-AuNCs in immature dendritic cells and its efficient in vitro labeling with BSA-AuNCs. In this work, we have developed protocols allowing an effective internalization of human monocyte-derived immature dendritic cells with red fluorescent BSA-AuNCs that are less toxic. Results infer that the high uptake efficiency of BSA coated AuNCs is related to their small size and to the nature of the ligand.
关键词： BSA-AuNCs,Cytotoxicity,Immature Dendritic Cells
更新于2025-09-10 09:29:36
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Fluorescent Neoglycoprotein Gold Nanoclusters: Synthesis and Applications in Plant Lectin Sensing and Cell Imaging
摘要： Carbohydrate-protein interactions mediate fundamental biological processes, such as fertilization, cell signaling, or host-pathogen communication. However, because of the enormous complexity of glycan recognition events, new tools enabling their analysis or applications emerge in recent years. Here, we describe the first preparation of neoglycoprotein functionalized fluorescent gold nanoclusters, containing a biantennary N-glycan G0 as targeting molecule, ovalbumin as carrier/model antigen, and a fluorescent gold core as imaging probe (G0-OVA-AuNCs). Subsequently, we demonstrate the utility of generated G0-OVA-AuNCs for specific sensing of plant lectins and in vitro imaging of dendritic cells.
关键词： Gold nanoclusters,Dendritic cells,Targeting,Neoglycoproteins,Lectin sensing,Carbohydrate-protein interactions
更新于2025-09-10 09:29:36